07/02/2023
Welcome to the first session of Downstream Processing! In this educational video, we will look at the critical features of the downstream processing recovery process, with a specific emphasis on cell disruption approaches.
Choosing the correct recovery procedure is critical for obtaining desired products from biological sources. We'll look at the aspects that influence this decision-making process, such as product characteristics, cost-effectiveness, and scalability. Understanding these factors will provide you with useful insights into optimising your downstream processing activities.
Following that, we'll look at the numerous types of cell disruption, which are essential for freeing intracellular components from cells. The following cell disruption strategies will be discussed:
Physical Methods: Learn how to use physical forces such as temperature, pressure, and osmotic shock to break open cells and liberate their contents. We'll look at methods including heat treatment, sonication, and high-pressure homogenization.
Explore mechanical approaches such as bead milling, high-speed homogenization, and high-pressure extrusion. Discover how these procedures effectively destroy cell walls and membranes, enabling efficient product recovery.
Chemical Methods: Investigate chemical substances capable of disrupting cell structures. We will discuss the application of enzymes, detergents, and organic solvents, emphasising their modes of action and applicability for certain applications.
Biological Methods: Investigate novel techniques to cell disruption that make use of biological agents. This section will include enzymatic therapies, viral lysis, and the use of microorganisms to aid in cell collapse.
By the end of this course, you will have a thorough understanding of the downstream processing recovery mechanism and the many cell disruption methods accessible. Join us on this trip as we provide you with the information and tools you'll need to run successful downstream processing operations.
Don't forget to like this video and subscribe to our channel if you want to learn more about downstream processing approaches. Stay tuned for Session 2, when we will look at further downstream processing processes, including as separation and purification procedures.
https://youtu.be/u_HB8myeE-I
Downstream Processing (Part 1) -Cell Disruption Methods - Intracellular Products | Dr. Deepika Malik Welcome to the first session of Downstream Processing! In this educational video, we will look at the critical features of the downstream processing recovery...
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