10/12/2026
What exactly is inside each file your pipeline produces? And do you know which one the next tool actually needs?
Our new blog is a practical guide to ๐๐ก๐๐-๐๐๐ช ๐๐ข๐ฅ๐ ๐๐จ๐ซ๐ฆ๐๐ญ๐ฌ, following the data from raw reads to peaks. It covers FASTQ, SAM/BAM, BED, ๐ง๐๐ซ๐ซ๐จ๐ฐ๐๐๐๐ค ๐๐ง๐ ๐๐ซ๐จ๐๐๐๐๐๐ค, ๐๐๐๐๐ซ๐๐ฉ๐ก ๐๐ง๐ ๐๐ข๐ ๐๐ข๐ , FASTA, GTF/GFF, and TSV/CSV, explaining what each file stores, which tools read it, and where it fits in the workflow. It also flags common mistakes, like mixing up ๐๐๐ ๐๐ง๐ ๐๐๐ or treating a signal track as a peak call.
Want to put these formats to work on real ๐๐ฎ๐ง๐ญ๐ข๐ง๐ ๐ญ๐จ๐ง'๐ฌ ๐๐ข๐ฌ๐๐๐ฌ๐ data? Details on our upcoming Epigenomics workshop are inside.
๐๐๐๐ ๐ญ๐ก๐ ๐๐ฎ๐ฅ๐ฅ ๐๐ฅ๐จ๐ ๐ก๐๐ซ๐:
https://omicslogic.com/blogs/from-reads-to-peaks-understanding-chip-seq-file-formats
Which file format tripped you up the most when you started working with NGS data?