The Upper Midwest Agricultural Safety and Health (UMASH) Center is one of eleven Centers of Excellence in Agricultural Disease and Injury Research, Education, and Prevention funded by the National Institute for Occupational Safety and Health (NIOSH) throughout the United States. The center is a collaboration of the University of Minnesota School of Public Health and College of Veterinary Medicine, the National Farm Medicine Center of the Marshfield Clinic, the Migrant Clinicians Network, and the Minnesota Department of Health. This collaboration brings together unique and complementary expertise to address existing and emerging occupational health and safety issues in agriculture. UMASH has a focus on health and safety related to animal agriculture production, which has been representative of cattle, hog, and poultry production in the region. UMASH embraces a One Health philosophy that recognizes the connections between human, animal, and environmental health when addressing occupational health and safety issues in agriculture. Research focus on livestock worker health and safety and zoonotic disease prevention has provided a framework for supporting farmers’ and farm workers’ health safety around livestock. Train-the-trainer programs for immigrant workers and rural firefighters build a system of safety training and practices by training trusted individuals or organizations. Agritourism research helps to manage risks associated with farm visitors. An emerging issues program supports efforts to identify and respond to new or re-emerging issues in agricultural safety and health. The center also has an outreach component to engage with the community, disseminate to and collect information from stakeholders, and build partnerships. The Upper Midwest Agricultural Safety and Health Center is funded by a cooperative agreement from the National Institute of Occupational Safety and Health, 1 U54 OH010170.