Citrus Greening

The updated preprint for the Asian citrus genome v3.0 is online https://www.biorxiv.org/content/10.1101/869685v2
with updated sections on transcription factors, s*x chromosome, educational impact and of course manual

The beneficial wasp Tamarixia radiata may be tiny, but has a large impact on saving California’s citrus. These tiny heroes are released throughout California to reduce populations of the Asian citrus psyllid, the pest that can spread Huanglongbing. Learn more about how these insects are getting the job done.

Prior transcriptome analyses of D. citri point to changes in biology due to CLas infection but have been hampered by an incomplete genome, proper host plant controls, and/or a lack of a uniform data analysis approach

https://academic.oup.com/gigascience/article/doi/10.1093/gigascience/giac035/6575386

The gut surface proteome of Diaphorina citri nymphs and adults is described. The authors compared the relative abundance of gut surface proteins for both adult and nymph D. citri, as nymphs are particularly susceptible to CLas infection.

Composition and abundance of midgut surface proteins in the Asian citrus psyllid, Diaphorina citri The Asian citrus psyllid, Diaphorina citri, is the vector of Candidatus Liberibacter asiaticus (CLas), the presumed causative agent of citrus greening…

"Developing solutions for citrus greening disease powered by the next generation of scientists" - Breaking Ground with Surya Saha - Boyce Thompson Institute Tuesday April 19, 12pm EST Citrus greening disease, or huanglongbing, is currently ravaging the US citrus industry. Researchers across the country are working tirelessly to discover methods to prevent and treat the disease, studying citrus plants, the bacterium that causes the disease, and the insec...

The authors show that the dominant parasitoid of ACP, Tamarixia radiata, can acquire the HLB pathogen Candidatus Liberibacter asiaticus (CLas) and transmit it horizontally when probing ACP nymphs. If these ACP nymphs survive the probing, develop to adults and move to healthy plants, CLas can be transmitted to citrus leaves during feeding. They illustrate the formerly unrecognized risk that a parasitoid can potentially serve as a phoretic vector of the pathogen transmitted by its host, thus potentially diminishing some of the benefits it confers via biocontrol.

https://www.nature.com/articles/s42003-021-02851-2

Interested in ag-related omics and project management? Research Associate position open in the Mueller lab
Major responsibilities will include leading genomics and transcriptomics analysis, genome annotation, code development and maintenance of citrus greening and agrivectors genomics databases and websites.
http://bti.hrmdirect.com/employment/job-opening.php?req=1765948&&

Authors report the identification of a ~8.3 kb DNA region of the Las genome containing eight putative open reading frames (ORFs) flanked by two inverted repeats, which was not present in the Las str. psy62 genome

Identification of a chromosomal deletion mutation and the dynamics of two major populations of Candidatus Liberibacter asiaticus in its hosts | Phytopathology® Candidatus Liberibacter asiaticus (Las) is the prominent species of Liberibacter associated with huanglongbing, a devastating disease of citrus worldwide. In this study, we report the identification of a ~8.3 kb DNA region of the Las genome containing eight putative open reading frames (ORFs) flanke...

A major characteristic of the order of Hemiptera is feeding on the phloem sap of their plant hosts. Phloem feeders face high osmotic stress between the gut lumen and hemolymph due to the high level of sucrose in phloem sap. Targeting the osmoregulation mechanisms in Diaphorina citri Kuwayama, which transmits ‘Candidatus Liberibacter asiaticus’, the putative causal agent of Huanglongbing in citrus may lead to an effective control strategy.

Silencing of Aquaporin Homologue Accumulates Uric Acid and Decreases the Lifespan of the Asian Citrus Psyllid, Diaphorina citri (Hemiptera: Liviidae) The Asian citrus psyllid, Diaphorina citri Kuwayama is devastating the citrus industry worldwide. It transmits ‘Candidatus Liberibacter asiaticus’, the pathogen of Huanglongbing in citrus. RNA interference is an excellent tool for functional genomics and for screening target genes for pest contr...

We have a new set of manual curations from the undergraduate driven Asian psyllid genome project!

Please check them out on biorxiv

Students from IRSC - Indian River State College Kansas State University Cornell University