Developmental Studies Hybridoma Bank

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Developmental Studies Hybridoma Bank Our mission is to provide the basic research community with hybridomas and monoclonal antibodies of the highest quality at the lowest prices.

The DSHB was created in 1986 by the Institute of Child Health and Human Development (NIH) to bank and distribute hybridomas and the monoclonal antibodies (MAbs) they produce to the general scientific community in order to facilitate research. Our first intention was, and still is, to keep the prices low so that researchers can test multiple MAbs without commitment of significant funds, then continue to utilize those of interest without worry of expense. A second intent was to relieve scientists of the time and expense of distributing hybridomas and MAbs they had developed. A third intent was to assure the scientific community that MAbs with limited demand would still remain available. Our fourth intent was to maintain the highest quality and to assist our customers in a timely fashion. We have striven over the past twenty-three years to remain true to our mission. Since 1996 we have been completely self-sufficient, requiring no funds from NIH, and have been able to keep prices extremely low. We intend to continue this policy.

New on the DSHBlog! Nearly four decades of shared discovery between DSHB and Mayo Clinic.That relationship began in 1987...
09/04/2026

New on the DSHBlog! Nearly four decades of shared discovery between DSHB and Mayo Clinic.

That relationship began in 1987, when J. A. McDonald deposited M-38, against collagen (pro) type I. In 1989, Jerry Katzmann added B4-78, against tissue-nonspecific alkaline phosphatase (AP-TNAP), an enzyme essential to bone mineralization.

Since then, Mayo Clinic researchers have put DSHB reagents to work across studies of muscle physiology, respiratory function, and neuromuscular disease. Gary Sieck’s Cell and Regenerative Physiology Lab alone has used 23 different DSHB antibodies, including in research on age-related vulnerability of respiratory motor neurons and their mitochondria.

Read the full story:

https://dshb.biology.uiowa.edu/blog =nearly-four-decades-of-discovery-mayo-clinic-and-the-dshb

What antibody question keeps coming up in your lab?Maybe it’s choosing between clones, predicting cross-species reactivi...
08/28/2026

What antibody question keeps coming up in your lab?

Maybe it’s choosing between clones, predicting cross-species reactivity, finding the right fixation conditions, or deciding what validation evidence is enough. The answer often lives in a lab notebook—or in the memory of the one person who tried everything.

The DSHBlog is our place to make more of that knowledge findable. We’ll share practical antibody guidance, research stories, behind-the-scenes looks at DSHB, and perspectives on reagent sharing and open science.

Tell us what would be useful at your bench. What should we cover next?

Read the DSHBlog: https://dshb.biology.uiowa.edu/blog

Every lab has its antibody lore.Someone knows which clone gives the cleanest signal. Someone else remembers the fixation...
08/24/2026

Every lab has its antibody lore.

Someone knows which clone gives the cleanest signal. Someone else remembers the fixation condition that finally worked. A protocol acquires notes in three different handwritings, and a trusted reagent moves from one project—and one generation of researchers—to the next.

That practical knowledge is too valuable to stay scattered. So we created the DSHBlog: a place for antibody know-how, research stories, technical guidance, repository news, and conversations about making scientific resources more accessible and useful.

We want the research community to help shape it. What antibody question comes up repeatedly in your lab? Which technique should we explore? Is there a DSHB reagent that made a difference in your research or teaching?

Welcome to the DSHBlog: https://dshb.biology.uiowa.edu/blog =welcome-to-the-dshb-blog-antibodies-answers-and-the-science-we-share

Does the DHDDS T206A mutation contribute to retinitis pigmentosa 59 on its own—or only in combination with K42E?Research...
08/21/2026

Does the DHDDS T206A mutation contribute to retinitis pigmentosa 59 on its own—or only in combination with K42E?

Researchers used knock-in mouse models carrying the mutations singly and together to examine retinal structure and function. Their results support T206A as independently pathogenic and add detail to how disruption of the dolichol-synthesis pathway can contribute to retinal degeneration.

Two DSHB antibodies—39.4D5 (RRID: AB_2314683) and PCRP-MEIS2-2B4 (RRID: AB_2618844)—helped identify and quantify losses in retinal interneurons, connecting cellular changes with functional impairment.

This is the kind of work in which careful cell identification turns a genetic result into a tissue-level story. Which retinal cell markers have been most useful in your research?

Read the story: https://dshb.biology.uiowa.edu/blog =using-dshb-antibodies-to-get-a-closer-look-at-the-genetics-of-vision-loss-

08/17/2026

Axons aren’t empty cables—and in a circuit that depends on fractions of a millisecond, the space inside them matters.

A study of the canary premotor song system combined electron microscopy with computational modeling to examine small, unmyelinated axons connecting the HVC and RA nuclei. Mitochondrial occupancy increased axial resistance and produced tiny delays in action-potential propagation across the pathway. Those delays fall on the same submillisecond timescale that precise birdsong requires.

DSHB mAb 3A10 (RRID: AB_531874) helped the researchers visualize and map the HVC→RA axon bundles; electron microscopy and modeling then addressed how mitochondrial occupancy affected signal timing.

It’s a striking reminder that cell biology and circuit physiology meet in the physical dimensions of an axon. Where else might organelle occupancy have meaningful timing consequences?

Read the story: https://dshb.biology.uiowa.edu/blog =powering-the-pathway-how-mitochondria-slow-the-signals-behind-birdsong

Will an antibody recognize your species? You won’t know for certain until you test it—but you can make the decision less...
08/14/2026

Will an antibody recognize your species? You won’t know for certain until you test it—but you can make the decision less of a blind gamble before you order.

Three free checks can help:

• Use BLAST to compare the target sequence in your species with the antigen species.
• If the epitope is known, use predicted structures for context: are sequence changes near the epitope, and might they alter its accessibility?
• Search Google Scholar for prior use of the clone, catalog name, or RRID in your species and application.

None of these replaces experimental validation, but together they can help you choose a stronger candidate and plan better controls.

What do you check before purchasing an antibody? Add your must-do step in the comments.

Read the guide: https://dshb.biology.uiowa.edu/blog =choosing-an-antibody-with-confidence

How can antibodies help illuminate genetic vision loss? In a study of retinitis pigmentosa type 59, researchers used DSH...
08/11/2026

How can antibodies help illuminate genetic vision loss? In a study of retinitis pigmentosa type 59, researchers used DSHB antibodies to identify retinal interneuron loss. Read more: https://ow.ly/Qqgb50ZyExf

08/07/2026

DSHB antibody 3A6B4 just celebrated its 20th anniversary in our inventory! Want to learn more about how this anti-Mmp1 antibody continues to further immune signaling and tumor research? Check out our latest blog post: https://dshb.biology.uiowa.edu/blog

What antibody question keeps coming up in your lab?Maybe it’s choosing between clones, predicting cross-species reactivi...
08/05/2026

What antibody question keeps coming up in your lab?

Maybe it’s choosing between clones, predicting cross-species reactivity, finding the right fixation conditions, or deciding what validation evidence is enough. The answer often lives in a lab notebook—or in the memory of the one person who tried everything.

The DSHBlog is our place to make more of that knowledge findable. We’ll share practical antibody guidance, research stories, behind-the-scenes looks at DSHB, and perspectives on reagent sharing and open science.

Tell us what would be useful at your bench. What should we cover next?

Read the DSHBlog:

08/05/2026

Every antibody has a story. Check out our blog to read the stories behind our antibodies, including the publications that rely on them and the institutions and researchers who develop them. https://dshb.biology.uiowa.edu/blog

Address

210 East Iowa Avenue
Iowa City, IA
52242

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Monday 8am - 5pm
Tuesday 8am - 5pm
Wednesday 8am - 5pm
Thursday 8am - 5pm
Friday 8am - 5pm

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+13193353826

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