08/04/2026
In celebration of National Water Quality month and to highlight the importance of karst groundwater we would like to share with you this cave science snack that investigates the movement of organic matter through karst aquifers:
Characterization of dissolved organic matter in cave and spring waters using UV–Vis absorbance and fluorescence spectroscopy by Justin E. Birdwell & Annette Summers Engel
https://doi.org/10.1016/j.orggeochem.2009.11.002
Birdwell and Engel (2010) demonstrated that combining ultraviolet-visible (UV–Vis) absorbance with fluorescence excitation-emission matrix (EEM) spectroscopy is a powerful way to characterize dissolved organic matter (DOM) in cave and spring waters. Their work showed that karst waters contain a mixture of terrestrial organic matter transported from surface soils and vegetation, as well as organic matter produced by microbial activity within the aquifer. Because DOM changes with recharge, flow paths, and microbial processing, fluorescence spectroscopy provides valuable insight into groundwater movement, carbon cycling, and water quality. This research gives us powerful tools to evaluate groundwater threats, monitor water quality and protect karst resources.
NCKRI uses fluorescence spectroscopy to trace carbon sources, biogeochemical processes, and contaminants in karst systems.
To learn more about National Water Quality Month:
https://nationalwaterqualitymonth.org/