Laboratorio de Investigación en Alimentos Funcionales-UNJFSC

On July 1, the following research “Storage stability of an antioxidant tea prepared from purple corn (Zea mays L.) cob and stevia (Stevia rebaudiana Bert.) and its effects on biomarkers of oxidative stress in healthy humans” was published in the Journal “Food Research International”. Impact Factor=8 and quartile of Q1 in Scopus. Link: https://www.sciencedirect.com/science/article/pii/S0963996925013201

Una nueva investigación publicada el 23 de agosto 2024: Microfluidic paper-based analytical device for measurement of pH using as sensor red cabbage anthocyanins and gum arabic.
https://www.sciencedirect.com/science/article/pii/S0308814624026141
https://doi.org/10.1016/j.foodchem.2024.140964

Un articulo científico más recientemente publicado, mi participación fue ente otras, la medición de la capacidad antioxidante por diferentes métodos. La revista Food Chemistry tiene un factor de impacto de 8.8 (ScienceDirect). La segunda fase de la investigación es lograr lo mismo y mejor pero con asistencia de Ultrasonido.

COLORIMETRY AND IMAGE ANALYSIS
Más información: https://bit.ly/3N4FBWj

Experiments at home. USE YOUR SMARTPHONE AS A SPECTROPHOTOMETER
Experimentos en casa. Use su celular como un espectrofotómetro

Smartphone "Spectrophotometer" This is a simple experimental set up that allows students to visualize the processes involved in spectrophotometric measurements. As a bonus, excellent resul...

Assessment of total antioxidant capacity of altiplano colored quinoa (Chenopodium quinoa willd) by visible and near-infrared diffuse reflectance spectroscopy and chemometrics.
Evaluación de la capacidad antioxidante total de quinua coloreada del altiplano (Chenopodium quinoa willd) por espectroscopia de reflectancia difusa visible e infrarroja cercana y quimiometría.

Quinoa (Chenopodium quinoa Willd.) seed has been recognized as an extremely nutritious grain, due to its content of protein, essential amino acids, essential fatty acids and minerals. In addition, quinoa is an important source of antioxidants and it has been used for medicinal purposes in humans. The high antioxidant capacity of quinoa is mainly the result of its phenolic and betalain content.
Thirty-eight colored quinoa seeds were collected from different provinces from the altiplano region of Puno (Peru), our aim in this study was to measure the TAC (Total antioxidant capacity) of altiplano colored quinoa using direct non-invasive Vis-NIR spectroscopy and chemometrics.
In conclusion; QUENCHER-DPPH results were 1.03–7.03 times higher than the EAC-DPPH values, which means that the non-extractable antioxidant capacity of altiplano colored quinoa represents some 2.8%–85.8% of the TAC. The best multivariate models to predict the TAC were obtained when the Vis-NIR spectral data were pre-processed using SG, DT and MSC scattering correction for whole quinoa seeds and SG, KM, SNV for ground quinoa. Thus, Vis-NIR spectroscopy and chemometric enable us to predict the TAC, including both the extractable and non-extractable fractions, in colored quinoa from the altiplano region.
https://www.sciencedirect.com/science/article/abs/pii/S0023643820311713

COLORIMETER (Chromameters KONICA MINOLTA CR-400).
The portable Colorimeter Cr-400, is used to measure the color parameters (generally, brightness, redness, and yellowness) of samples in many different applications including paints, inks, plastics, textiles, food, plants, beverages, pharmaceuticals, and cosmetics. The chromameter uses the tristimulus color-sensing method based on the 3-component theory of color vision for the human eye, which senses 3 primary colors (red, green, and blue) and perceives all colors as mixtures of these 3 primary colors. Ideal for measuring reflected color and color difference of ingredients, fruits, grains, raw materials, finished products, powders, pastes, and opaque liquids. The measuring head is equipped with Display, function keys and power supply, can be used as "stand alone" without the Data Processor or as an additional option even directly be interfaced to the PC. Included Accessories and data processor, operating; Illuminant C and D65, several color spaces (CIELAB, XYZ, Yxy, Hunter Lab, L*C*h, and others), simple calibration, good usability and functionality in foods.

ROTATIONAL VISCOMETER
EVO Expert, FUNGILAB. provides rapid measurements of medium viscosity samples (ideal to the rheological property in oils, juices, yogurt, and others).
defined step, ramp, and multi-step curves can be programmed, and a powerful graph key helps to design the flow curves needed. Up to four flow curves can be compared simultaneously, and over twelve different charts can be produced. The software also allows the documentation of each experiment with a name, date/time stamp, user, and notes for performing follow-up experiments. The data logger function allows data to be transferred in a spreadsheet format to a PC or sent directly to a local printer via the USB drive.
Operating Viscosity range: 100cP to 40 million cP, Temperature range: -40°C – 300°C (-40°F –572°F), RTD temperature probe. Viscosity reading in cP (mPa·s) or cSt, Model EVO EXPERT L, Rango (Cp) 1 - 6.000.000, speed (RPM); 0.01 - 250, number of speeds 2600. AISI 316 Stainless steel spindle is easy to identify according to its letter and number, in terms of the range of viscosity.

UHPLC system ultimate 3000 of thermo scientific, provides excellent chromatographic performance while maintaining easy-to-use, reliable operation. Configured with; Diode array detector (DAD), Refractive index (RI) and Charged aerosol detector (CAD).Includy Thermo Scientific Chromeleon Chromatography Data System (CDS) software.

The IS50 FT-IR Spectrometer
The Thermo Scientific Nicolet iS50 FTIR Spectrometer is the all-in-one materials analysis workstation. System that can acquire spectra from far-infrared to visible, easy is getting the spectral footprint in foods, polymers, feed, soils, rubbers, pharmaceuticals, forensics or any other materials.
We is used in laboratory to acquire data utilizing ATR in the both the mid and far-IR regions, as well as near-IR spectroscopy, the Fourier transform near-infrared (FT-NIR) or Near-infrared spectroscopy (NIRS) is a fast and non-destructive analytical method, Associated with chemometrics, you can developed for simple analysis of anthocyanin, flavonoid, carotenoids, glucosinolates, total sugar, total acid, total phenolic content, and total antioxidant activity. Major classes of other bioactive compounds, including pre- and probiotics, is can to be analysed using NIR spectroscopy. The use of NIR spectroscopy for analysis of bioactive compounds is expected to match the growth of the functional food and bioactive ingredients markets.

PHOTOCHEM® - ANALYTIK JENA; Measurement of Water-soluble and Lipid-soluble Antioxidative Capacity.
The PHOTOCHEM apparatus of Analytik Jena (Germany) is used to measure the antioxidant activity whit the PCL assay (photochemiluminescence). The PLC assay is easy and rapid to perform and has many advantages over other assays. It does not require high temperatures to generate radicals and it is more sensitive (nanomolare range) to measure within few minutes (