Communauté générale d’éducation numérique

Communauté générale d’éducation numérique

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CGEN est un groupement des gens qui développent,améliorent et partagent en permanence des nouvelles techniques de compréhensions et d'éducations numériques

medical and biological databases 14/10/2019

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08/03/2019

High level connectivity using mapping technology white matter fiber

08/03/2019

Thalamo-cortico connectivity with MRI using tracking lines

Photos from Communauté générale d’éducation numérique's post 18/02/2019

And another batch of trans-[Co(en)2Cl2]Cl, this time using a different method that yields a purer product

17/02/2019

Neurography of cervical spine and nerve roots with 1.5 tesla

14/02/2019

High resolution MRI of MSK 3D MERGE/MEDIC with and without water excite fat sat

12/02/2019

Cortical circuits consist of precise cell-type specific connections, whereby subcortical brain structures form connections with specific cortical inhibitory neuron subtypes

12/02/2019

NRSE and a minimal CMV promoter showed markedly restricted expression to a small subset of mostly inhibitory neurons, but whose commonalities are unknown.

10/02/2019

Fertility in mammals is controlled by hypothalamic neurons that secrete gonadotropin-releasing hormone (GnRH). These neurons differentiate in the olfactory placodes during embryogenesis and migrate from the nose to the hypothalamus before birth. Information regarding this process in humans is sparse. Here, we adapted new tissue-clearing and whole-mount immunohistochemical techniques to entire human embryos/fetuses to meticulously study this system during the first trimester of gestation in the largest series of human fetuses examined to date. Combining these cutting-edge techniques with conventional immunohistochemistry, we provide the first chronological and quantitative analysis of GnRH neuron origins, differentiation and migration, as well as a 3D atlas of their distribution in the fetal brain. We reveal not only that the number of GnRH-immunoreactive neurons in humans is significantly higher than previously thought, but that GnRH cells migrate into several extrahypothalamic brain regions in addition to the hypothalamus. Their presence in these areas raises the possibility that GnRH has non-reproductive roles, creating new avenues for research on GnRH functions in cognitive, behavioral and physiological processes.

10/02/2019

Whole mouse-brain vasculature tomography. (a) 3D rendering (Amira 5.3 software, Visage Imaging) of the whole mouse-brain vasculature acquired using LSFM and gel-BSA-FITC vascular staining. The image was produced from downsampled stacks stitched using the TeraStitcher software 42. (b) Single frame from a stack at original resolution (pixel size 0.65 ?m) showing details at the capillary level in a very internal optical section of the brain.

10/02/2019

Whole mouse brain neuronal and vascular imaging. (left) (a) Whole-brain 3D rendering of GFPexpressing neurons acquired with LSFM. (b) 3D rendering of the same brain showing blood vessels labeled with gel-BSA-FITC acquired with LSFM. Downsampled stacks from LSFM acquisitions were stitched using TeraStitcher software42. (c) Composite image. (right) 100 um MIPs from original acquisitions (z-step 2 um, xy pixel size 0.65 ?m). (a) GFP-expressing neurons; (b) gel-BSA-FITC labelled vasculature; (c) composite.

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