新学術領域「運動マシナリー」総括班による先端的電子顕微鏡観察法の開発

新しい論文が出版されました．運動マシナリー領域が終わってから大阪市大内のプロジェクトとして行われたものです．今はやりの細菌膜小胞（Bacterial MV）の論文です．論文では，急速凍結ディープエッチレプリカの像を沢山採用していただきました．
https://www.frontiersin.org/articles/10.3389/fmicb.2021.706525/full?fbclid=IwAR0iy9sCPQe7l-T-p_RRYdVya2xg-YpzyiA1iZLRNrXoBWvRKHkOu9TW6Sk

そしてこの論文では雑誌の論文賞もいただきました．田原研究員がつくばの学会で受賞講演をしました．http://microscopy.or.jp/about/award/paper/

Peptidoglycan layer and disruption processes in Bacillus subtilis cells visualized using quick-freeze, deep-etch electron microscopy Abstract. Peptidoglycan, which is the main component of the bacterial cell wall, is a heterogeneous polymer of glycan strands cross-linked with short peptides a

共同研究大募集です．

Involvement of a Multidrug Efflux Pump and Alterations in Cell Surface Structure in the Synergistic Antifungal Activity of Nagilactone E and Anethole against Budding Yeast Saccharomyces cerevisiae Nagilactone E, an antifungal agent derived from the root bark of Podocarpus nagi, inhibits 1,3-β glucan synthesis; however, its inhibitory activity is weak. Anethole, the principal component of anise oil, enhances the antifungal activity of nagilactone E. We aimed to determine the combinatorial eff...

濡れ手に粟です．

Mycolic acid-containing bacteria trigger distinct types of membrane vesicles through different routes Bacterial membrane vesicles (MVs) are attracting considerable attention in diverse fields of life science and biotechnology due to their potential for…

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Quick-Freeze, Deep-Etch Electron Microscopy Reveals the Characteristic Architecture of the Fission Yeast Spore The spore of the fission yeast Schizosaccharomyces pombe is a dormant cell that is resistant to a variety of environmental stresses. The S. pombe spore is coated by a proteinaceous surface layer, termed the Isp3 layer because it comprises mainly Isp3 protein. Although thin-section electron microscop...

急速凍結ディープエッチレプリカ法は，微生物では，菌類の構造一般，細菌のペプチドグリカン層，細菌の膜小胞の解析に極めて有効であることがわかってきました．論文もどんどん発表しています．MDPIもありますが，そんなことは気にしません．

The Fission Yeast RNA-Binding Protein Meu5 Is Involved in Outer Forespore Membrane Breakdown during Spore Formation In Schizosaccharomyces pombe, the spore wall confers strong resistance against external stress. During meiosis II, the double-layered intracellular forespore membrane (FSM) forms de novo and encapsulates the nucleus. Eventually, the inner FSM layer becomes the plasma membrane of the spore, while the...

静岡大の田代陽介さんたちと急速凍結ディープエッチレプリカ電子顕微鏡で撮影したButtiauxella agrestisの膜小胞の画像がApplied and Environmental Microbiologyに掲載され，表紙に選ばれました．https://aem.asm.org/content/86/20.cover-expansion

急速凍結レプリカ法ではありませんが，領域運動マシナリーのテーマだった研究がやっとNature Communicationsで発表できました．肺炎マイコプラズマの感染の全ての中心で働くタンパク質複合体です．この構造はマイコプラズマ学40年来の夢でもありました．これからは，接着，滑走，抗原性変化を具体的に考えることができます．

Immunodominant proteins P1 and P40/P90 from human pathogen Mycoplasma pneumoniae Adhesion of the human pathogen Mycoplasma pneumoniae to pulmonary epithelial cells is mediated by a transmembrane complex composed of proteins P1 and P40/P90. Here, the authors present the structures of M. pneumoniae P1 and P40/P90, show that P40/P90 binds sialylated oligosaccharides and have also d...

Tidy up cryo-EM sample grids with 3D printed tools Cryo-EM technology has developed to the point of high-throughput structure determination of biological macromolecules embedded in vitreous ice. Noneth…

Peptidoglycan layer and disruption processes in Bacillus subtilis cells visualized using quick-freeze, deep-etch electron microscopy Abstract. Peptidoglycan, which is the main component of the bacterial cell wall, is a heterogeneous polymer of glycan strands cross-linked with short peptides

枯草菌のペプチドグリカンとその崩壊過程を急速凍結レプリカ法で可視化した論文を発表します．本日投稿予定ですが，先だってbioRxivで公開しました．コメントや拡散などをお願いできればと思います．
Peptidoglycan layer and disruption processes in Bacillus subtilis cells visualized using quick-freeze, deep-etch electron microscopy
Isil Tulum, Yuhei O Tahara, Makoto Miyata
https://www.biorxiv.org/content/10.1101/600171v1

塩見大輔 准教授（立教大）
と，うちの卒業生の笠井大司助教，4年生の近田大基さんの3人が大腸菌のL-formを観察するために，訪問されました．セミナーとパーティーも盛り上がりました．たくさんの研究のヒントも得られました．