Chromagar

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CHROMagar™ StrepB

For isolation and differentiation of Streptococcus agalactiae (GBS)

Medium Performance

Compared to CNA Blood Agar and Granada:

Easy interpretation: easier reading thanks to an intense and sensitive mauve colony colouration.

High sensitivity: detection of GBS, including non-haemolytic strains, with sensitivity close to 100 %.

High selectivity: differenciation of GBS from other bacteria by selective inhibition or by counter-colouration.

Fast: results in 18-24 h.

Simplicity: incubation in aerobic conditions. Confirmation by latex agglutination can be performed directly on the colony.

Typical Appearance of microrganisms
Streptococcus B –› Mauve
Other Microorganism
→ blue, colourless or inhibited

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CHROMagar™ Salmonella

For detection and isolation of Salmonella spp.,
including S. typhi and S. paratyphi
in clinical specimens.

Medium Performance

Easy reading: intense mauve colony colours for better identification.
Greater Specificity / Less workload: conventional media for the detection of Salmonella by H2S character have very poor specificity resulting in numerous false positives (Citrobacter, Proteus, etc.) among the rare, real positive Salmonella. The workload for unnecessary examination of suspect colonies is so heavy that real positive Salmonella colonies might often be overlooked in routine testing. Because of their poor specificity, conventional media require a tedious examination of at least 10 colonies per suspected sample.
On the contrary, CHROMagar Salmonella eliminates most of those false positives and allows technicians to focus on the real contaminated samples.
High sensitivity, leading to a higher detection rate of Salmonella:
Salmonella sensitivity: 95%*
Specificity: 88,9%* compared to 78% with Hektoen Agar.
*Specificity and sensitivity from scientific study: “Comparison of CHROMagar Salmonella medium and Hektoen Enteric Agar for isolation of Salmonellae from stool samples.” Gaillot O. et al. 1999. Journal of Clinical Microbiology, 37 : 762-765
Dramatic decrease of the workload:
Number of useless confirmatory tests is minimized since there is no need of duplicating them.

Typical Appearance of microrganisms
Salmonella → mauve
Other bacteria → blue, colourless, or inhibited.

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CHROMagar™ Orientation

For isolation and differentiation
of urinary tract pathogens.

Medium Performance

Isolation of a variety of microorganisms: the major target of this medium is the detection of urinary tract pathogens but CHROMagarTM Orientation has a broader application when supplemented with various antibiotics in detecting increasingly important nosocomial and multi-drug resistant microorganisms (See CHROMagarTM ESBL, CHROMagarTM KPC supplements).

It presents an instant palette of colours to obtain a large spectrum of differentiation of species. CHROMagarTM Orientation has several advantages over traditional media:
- allows in most cases full differentiation of the pathogens
- allows for reliable detection semi quantitative and presumptive identification of urinary tract pathogens
- easier recognition of mixed growth
- provides higher detection rates

High detection of minor populations: the proper use of CHROMagarTM Orientation will correctly pinpoint the presence of a minor population and will help to establish the right diagnosis and therapy.

Save time and reduce workload thanks to a high specificity: The most common UTI pathogen is E. coli, found in 40-70 % of infections. CHROMagarTM Orientation has a specificity of 99,3 %* for E. coli, rendering the species confirmatory test largely unnecessary.
* Merlino, J. et al. 1996. Evaluation of CHROMagar Orientation for Differentiation and Presumptive Identification of Gram-Negative Bacilli and Enterococcus Species, J.C.M. 34: 1788-1793.

One plate of CHROMagarTM Orientation will give the same information as the combination of the 3 classical plates used for UTI analysis (blood agar, CLED and MacConkey agar). Moreover since it is easy to differentiate mixed flora on CHROMagarTM Orientation, antimicrobial susceptibility tests can be performed directly from primary isolates without the need of subcultures.
* Samra, Z. et al. 1998. Evaluation of use of a new chromogenic agar in detection of urinary tract pathogens, J.C.M. 36: 990-994.

Typical Appearance of microrganisms
E. coli → dark pink to reddish
Enterococcus → turquoise blue
Klebsiella, Enterobacter, Citrobacter → metallic blue
Proteus → brown halo
Pseudomonas → cream, translucent
S. aureus → golden, opaque, small
S. saprophyticus → pink, opaque, small

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CHROMagar™ Campylobacter

For Detection of thermotolerant Campylobacter

Medium Performance

IN ACCORDANCE TO ISO 10272-1 REQUIREMENTS
as a selective medium for the detection of Campylobacter

EASY READING
36-48 h incubation at 42 °C. The intense red colored colonies on a translucent agar facilitates the reading compared to charcoal based agar.

HIGH RECOVERY RATE ~100 %
enumerating Campylobacter has never been so simple and so reliable.

HIGHLY SELECTIVE
In addition, a reinforced selectivity allows for a very clean plate, even with heavily flora loaded samples.

Typical Appearance of microorganisms
Campylobacter jejuni, coli, lari → red
Other Bacteria → blue or inhibited

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CHROMagar™ Serratia

For detection of Serratia marcescens

Medium Performance

1. Reliable: First commercially available chromogenic medium for Serratia marcescens.

2. Very high sensitivity and selectivity: more than 97 %* for Serratia marcescens.
* Data obtained from the study "Validation of Colorex™ (CHROMagar™) Serratia agar on WASP™/WASPLab™ in screening for Serratia marcescens in neonatal intensive care units using the ESwab™ » M. Gaskin, D. Yamamura, J. Korver, 2020"

3. High specificity and lower workload: The conventional media used for the detection of S. marcescens like MacConkey Agar has very poor specificity, creating an abundance of false positives. Because all lactose negative bacteria have a similar aspect than S. marcescens. On the contrary, CHROMagarTM Serratia detect all Serratia (those pigmented or not) and allows technicians to focus on the real contaminated samples.

4. Intense green-blue colouration for easy reading, compared to conventional medium (MacConkey, TSA...).

5. Fast results particularly useful in case of sudden, dangerous outbreak of Serratia marcescens.

Typical Appearance of microrganisms
Serratia marcescens → blue-green to metallic blue

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CHROMagar™ Candida Plus

For detection and differentiation of major clinical Candida species, including C. auris

Medium Performance
1. High Specificity: Differentiation of the most common Candida species with very high specificity:
C. albicans ≈ 100 %*
C. tropicalis ≈ 100 %*
C. krusei ≈ 100 %*

2. Unique medium to differentiate C. auris from other Candida species: Owe to its high specificity, it can be used also as a screening tool in case of outbreaks.
Specificity ≈ 100 %*
Sensitivity ≈ 100 %*
* Specificity and Sensitivity from scientific study: «Evaluation of a novel chromogenic medium for Candida spp. identification and comparison with CHROMagarTM Candida for the detection of Candida auris in surveillance samples» Juan V. Mulet and al., 2020.

3. Easy identification: Identification by MALDI-TOF can be carried directly from a colony. No need of subculture.

Typical Appearance of microorganisms
Candida albicans → green-blue
Candida auris → light blue with blue halo
Candida tropicalis → metallic blue with pink halo
Candida krusei → pink and fuzzy

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