18/11/2020
CHROMagar™ Orientation
For isolation and differentiation
of urinary tract pathogens.
Medium Performance
Isolation of a variety of microorganisms: the major target of this medium is the detection of urinary tract pathogens but CHROMagarTM Orientation has a broader application when supplemented with various antibiotics in detecting increasingly important nosocomial and multi-drug resistant microorganisms (See CHROMagarTM ESBL, CHROMagarTM KPC supplements).
It presents an instant palette of colours to obtain a large spectrum of differentiation of species. CHROMagarTM Orientation has several advantages over traditional media:
- allows in most cases full differentiation of the pathogens
- allows for reliable detection semi quantitative and presumptive identification of urinary tract pathogens
- easier recognition of mixed growth
- provides higher detection rates
High detection of minor populations: the proper use of CHROMagarTM Orientation will correctly pinpoint the presence of a minor population and will help to establish the right diagnosis and therapy.
Save time and reduce workload thanks to a high specificity: The most common UTI pathogen is E. coli, found in 40-70 % of infections. CHROMagarTM Orientation has a specificity of 99,3 %* for E. coli, rendering the species confirmatory test largely unnecessary.
* Merlino, J. et al. 1996. Evaluation of CHROMagar Orientation for Differentiation and Presumptive Identification of Gram-Negative Bacilli and Enterococcus Species, J.C.M. 34: 1788-1793.
One plate of CHROMagarTM Orientation will give the same information as the combination of the 3 classical plates used for UTI analysis (blood agar, CLED and MacConkey agar). Moreover since it is easy to differentiate mixed flora on CHROMagarTM Orientation, antimicrobial susceptibility tests can be performed directly from primary isolates without the need of subcultures.
* Samra, Z. et al. 1998. Evaluation of use of a new chromogenic agar in detection of urinary tract pathogens, J.C.M. 36: 990-994.
Typical Appearance of microrganisms
E. coli → dark pink to reddish
Enterococcus → turquoise blue
Klebsiella, Enterobacter, Citrobacter → metallic blue
Proteus → brown halo
Pseudomonas → cream, translucent
S. aureus → golden, opaque, small
S. saprophyticus → pink, opaque, small
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