Eugenicslifesciences

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Summarize Molecular biology Techniques for CSIR B part, DBT JRF, ICMR, GATE techniques - B part you can WhatsApp Us for PDF of these ...

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CSIR NET Life Sciences June 2022 Morning Shift Questions, that will increase your marks -NET June 2022 2022 morning shift # increase your marks

New Six months Class room programme announcements

New batch for CSIR-NET Life Sciences for Dec 2022 is going to start from 14- June 2022

Boost Your Knowledge With Team Eugenics !
Announcement:- We are going to conduct Full length CSIR Based test series. Which includes 12 Full length subject-wise tests and 3 Mock test (Total 15 test). These test series will be divided into 3 parts:-
Part-A :- 20 questions, general Aptitude
Part-B :- 50 questions
Part-C:- 75 questions
Test series costs only RS 200/-
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Time table for test series is shown below

MiRNA Biogenesis

MicroRNAs (miRNAs) are a class of non-coding RNAs that play important roles in regulating gene expression. Canonical miRNA biogenesis begins with the generation of the pri-miRNA transcript. The microprocessor complex, comprised of Drosha and DiGeorge Syndrome Critical Region 8 (DGCR8), cleaves the pri-miRNA to produce the precursor-miRNA (pre-miRNA). The pre-miRNA is exported to the cytoplasm in an Exportin5/RanGTP-dependent manner and processed to produce the mature miRNA duplex. Finally, either the 5p or 3p strands of the mature miRNA duplex is loaded into the Argonaute (AGO) family of proteins to form a miRNA-induced silencing complex (miRISC). In the non-canonical pathways, small hairpin RNA (shRNA) are initially cleaved by the microprocessor complex and exported to the cytoplasm via Exportin5/RanGTP. They are further processed via AGO2-dependent, but Dicer-independent, cleavage. Mirtrons and 7-methylguanine capped (m7G)-pre-miRNA are dependent on Dicer to complete their cytoplasmic maturation, but they differ in their nucleocytoplasmic shuttling. Mirtrons are exported via Exportin5/RanGTP while m7G-pre-miRNA are exported via Exportin1. All pathways ultimately lead to a functional miRISC complex. In most cases, miRISC binds to target mRNAs to induce translational inhibition, most likely by interfering with the eIF4F complex. Next, GW182 family proteins bound to Argonaute recruit the poly(A)-deadenylases PAN2/3 and CCR4-NOT. PAN2/3 initiates deadenylation while the CCR4-NOT complex completes the process, leading to removal of the m7G cap on target mRNA by the decapping complex. Decapped mRNA may then undergo 5′−3′ degradation via the exoribonuclease XRN1.

Topic of the day.

MicroRNAs are small, endogenous, highly conserved non-coding RNA molecules involved in the regulation of gene expression. MicroRNAs are transcribed by RNA polymerases II and III, generating precursors that undergo a series of cleavage events to form mature microRNA.onsists of two cleavage events, one nuclear and one cytoplasmic. The regulatory functions of microRNAs are accomplished through the RNA-induced silencing complex (RISC). MicroRNA assembles into RISC, activating the complex to target messenger RNA (mRNA) specified by the microRNA. Various RISC assembly models have been proposed and research continues to explore the mechanism(s) of RISC loading and activation. The degree and nature of the complementarity between the microRNA and target determine the gene silencing mechanism, slicer-dependent mRNA degradation or slicer-independent translation inhibition. P-bodies are essential for microRNA-mediated gene silencing and that RISC assembly and silencing occurs primarily within P-bodies.