Sheo Mohan Singh

Happy Holi Everyone!

1-Week Hands-on Workshop on Human/Cancer Cell Culture Techniques & MTT Assay, Delhi. Next: 24-30 March 2025, click on https://icsccb.org/workshops/cellcultureworkshop/ to apply.

Following techniques will be done hands-on by each candidate using Human Embryonic Kidney-293T and Jurkat cell lines:

1. MTT Assay for cell proliferation/cytotoxicity
2. Cell culture techniques, passaging and maintenance of human cancer cell lines
3. Media preparation and counting of cells
4. Cryopreservation: Freezing & thawing of human cancer cell lines
5. Genomic DNA isolation from cancer cell lines,
6. Genomic DNA estimation
7. Genomic DNA agarose gel electrophoresis
8. Total RNA extraction from human cancer cell lines
9. cDNA synthesis from RNA (Reverse Transcription),
10. Polymerase Chain Reaction from cDNA (Reverse Transcription-PCR)
11. Agarose gel electrophoresis of PCR product
12. Total protein extraction from human cancer cell lines
13. Protein estimation
14. SDS-PAGE electrophoresis of extracted proteins
15. Coomassie blue staining of protein gel

This workshop is designed for beginners of all ages who wants to become confidant in lab techniques. This is a practical hands-on workshop to train the candidates in animal cell culture, molecular biology techniques and MTT assay using the human embryonic and cancer cell lines for various experiments. It is supported by lectures on cell culture techniques, molecular biology assays, MTT assay, gene/protein expression/interaction networks in hematopoietic stem cells (HSCs) and cancer cells.

6-Days Hands-on Workshop on Molecular Biotechnology, PCR and SDS-PAGE, Delhi. Next: 5-10 May 2025. Click on https://icsccb.org/workshops/biotech-workshop/ to apply.

Following techniques will be done real hands-on by each candidate:
Recombinant DNA Technology Lab:
1. Plasmid DNA isolation
2. Plasmid DNA agarose gel electrophoresis
3. DNA extraction from agarose gel
4. Restriction digestion of DNA
5. Ligation of DNA
6. Agarose gel electrophoresis of digested and ligated DNA
7. Preparation of competent cells
8. Transformation of plasmid DNA into competent cells
9. Plating of transformed cells on LB Agar plates
10. Polymerase Chain Reaction (PCR)
11. Agarose gel electrophoresis of PCR product
12. SDS-PAGE electrophoresis of proteins
13. Coomassie staining of protein gel

Bioinformatics tools for the above techniques:
1. DNA databases
2. Sequence Analysis: Pairwise sequence alignment, Multiple sequence alignment, BLAST tools
3. Designing of PCR primers,
4. Restriction analysis, restriction maps, plasmid maps,
5. Searching for homologous and paralogous sequences,
6. Promoter analysis,
7. Whole genome data visualisation: browsing genomic data with the help of NCBI Genome viewer or UCSC Genome browser.

This workshop is designed for beginners of all ages who wants to become confidant in lab techniques. It is a real hands-on training on the topics mentioned. Major focus is on the lab practicals. It will be supported by lectures in such a manner that it would help a biologist to understand the bioinformatics tools helpful in the lab and a bioinformatics person to understand the practical biotech lab techniques.

Nice article.

Nature Reviews Immunology: The peripheral immune system communicates with the brain through diverse anatomical routes to shape brain physiology. This Perspective discusses why such diversity is needed and explore how these routes are leveraged during development and hijacked in ageing.

Link to the Perspective in the comments.

1-Week Hands-on Workshop on Human/Cancer Cell Culture Techniques & MTT Assay, Delhi. Next: 24-30 March 2025, click on https://icsccb.org/workshops/cellcultureworkshop/ to apply.

Following techniques will be done hands-on by each candidate using Human Embryonic Kidney-293T and Jurkat cell lines:

1. MTT Assay for cell proliferation/cytotoxicity
2. Cell culture techniques, passaging and maintenance of human cancer cell lines
3. Media preparation and counting of cells
4. Cryopreservation: Freezing & thawing of human cancer cell lines
5. Genomic DNA isolation from cancer cell lines,
6. Genomic DNA estimation
7. Genomic DNA agarose gel electrophoresis
8. Total RNA extraction from human cancer cell lines
9. cDNA synthesis from RNA (Reverse Transcription),
10. Polymerase Chain Reaction from cDNA (Reverse Transcription-PCR)
11. Agarose gel electrophoresis of PCR product
12. Total protein extraction from human cancer cell lines
13. Protein estimation
14. SDS-PAGE electrophoresis of extracted proteins
15. Coomassie blue staining of protein gel

This workshop is designed for beginners of all ages who wants to become confidant in lab techniques. This is a practical hands-on workshop to train the candidates in animal cell culture, molecular biology techniques and MTT assay using the human embryonic and cancer cell lines for various experiments. It is supported by lectures on cell culture techniques, molecular biology assays, MTT assay, gene/protein expression/interaction networks in hematopoietic stem cells (HSCs) and cancer cells.

Hands-on Workshop on Molecular Biotechnology, PCR and SDS-PAGE, Delhi. Next: 5-10 May 2025. Click on https://icsccb.org/workshops/biotech-workshop/ to apply.

Following techniques will be done real hands-on by each candidate:
Recombinant DNA Technology Lab:
1. Plasmid DNA isolation
2. Plasmid DNA agarose gel electrophoresis
3. DNA extraction from agarose gel
4. Restriction digestion of DNA
5. Ligation of DNA
6. Agarose gel electrophoresis of digested and ligated DNA
7. Preparation of competent cells
8. Transformation of plasmid DNA into competent cells
9. Plating of transformed cells on LB Agar plates
10. Polymerase Chain Reaction (PCR)
11. Agarose gel electrophoresis of PCR product
12. SDS-PAGE electrophoresis of proteins
13. Coomassie staining of protein gel

Bioinformatics tools for the above techniques:
1. DNA databases
2. Sequence Analysis: Pairwise sequence alignment, Multiple sequence alignment, BLAST tools
3. Designing of PCR primers,
4. Restriction analysis, restriction maps, plasmid maps,
5. Searching for homologous and paralogous sequences,
6. Promoter analysis,
7. Whole genome data visualisation: browsing genomic data with the help of NCBI Genome viewer or UCSC Genome browser.

This workshop is designed for beginners of all ages who wants to become confidant in lab techniques. It is a real hands-on training on the topics mentioned. Major focus is on the lab practicals. It will be supported by lectures in such a manner that it would help a biologist to understand the bioinformatics tools helpful in the lab and a bioinformatics person to understand the practical biotech lab techniques.

Hands-on Workshop on Molecular Biotechnology, PCR and SDS-PAGE, Delhi, from 5-10 May 2025, click on https://icsccb.org/workshops/biotech-workshop/ to apply.

Following techniques will be done real hands-on by each candidate:
Recombinant DNA Technology Lab:
1. Polymerase Chain Reaction (PCR)
2. Agarose gel electrophoresis of PCR product
3. SDS-PAGE electrophoresis of proteins
4. Coomassie staining of protein gel
5. Restriction digestion of DNA
6. Ligation of DNA
7. Agarose gel electrophoresis of digested and ligated DNA
8. Preparation of competent cells
9. Transformation of plasmid DNA into competent cells
10. Plating of transformed cells on LB Agar plates
11. Plasmid DNA isolation
12. Plasmid DNA agarose gel electrophoresis
13. DNA extraction from agarose gel

Bioinformatics tools for the above techniques:
1. DNA databases
2. Sequence Analysis: Pairwise sequence alignment, Multiple sequence alignment, BLAST tools
3. Designing of PCR primers,
4. Restriction analysis, restriction maps, plasmid maps,
5. Searching for homologous and paralogous sequences,
6. Promoter analysis,
7. Whole genome data visualisation: browsing genomic data with the help of NCBI Genome viewer or UCSC Genome browser.

This workshop is designed for beginners of all ages who wants to become confidant in lab techniques. It is a real hands-on training on the topics mentioned. Major focus is on the lab practicals. It will be supported by lectures in such a manner that it would help a biologist to understand the bioinformatics tools helpful in the lab and a bioinformatics person to understand the practical biotech lab techniques.

1-Week Hands-on Workshop on Human/Cancer Cell Culture Techniques & MTT Assay. Delhi, 24-30 Mar 2025, click on https://icsccb.org/workshops/cellcultureworkshop/ to apply.

Following techniques will be done hands-on by each candidate using Human Embryonic Kidney-293T and Jurkat cell lines:

1. MTT Assay for cell proliferation/cytotoxicity
2. Cell culture techniques, passaging and maintenance of human cancer cell lines
3. Media preparation and counting of cells
4. Cryopreservation: Freezing & thawing of human cancer cell lines
5. Genomic DNA isolation from cancer cell lines,
6. Genomic DNA estimation
7. Genomic DNA agarose gel electrophoresis
8. Total RNA extraction from human cancer cell lines
9. cDNA synthesis from RNA (Reverse Transcription),
10. Polymerase Chain Reaction from cDNA (Reverse Transcription-PCR)
11. Agarose gel electrophoresis of PCR product
12. Total protein extraction from human cancer cell lines
13. Protein estimation
14. SDS-PAGE electrophoresis of extracted proteins
15. Coomassie blue staining of protein gel

This workshop is designed for beginners of all ages who wants to become confidant in lab techniques. This is a practical hands-on workshop to train the candidates in animal cell culture, molecular biology techniques and MTT assay using the human embryonic and cancer cell lines for various experiments. It is supported by lectures on cell culture techniques, molecular biology assays, MTT assay, gene/protein expression/interaction networks in hematopoietic stem cells (HSCs) and cancer cells.

Very informative.

Telomere function and regulation from mouse models to human ageing and disease Nature Reviews Molecular Cell Biology

Nice work.

Sulforaphane, found in broccoli sprouts, has been shown to reduce blood sugar levels in individuals with prediabetes – according to a study published in Nature Microbiology.

Link to the research in the comments.

1-Week Hands-on Workshop on Human/Cancer Cell Culture Techniques & MTT Assay. Delhi, 24-30 March 2025, click on https://icsccb.org/workshops/cellcultureworkshop/ to apply.

Following techniques will be done hands-on by each candidate using Human Embryonic Kidney-293T and Jurkat cell lines:

1. MTT Assay for cell proliferation/cytotoxicity
2. Cell culture techniques, passaging and maintenance of human cancer cell lines
3. Media preparation and counting of cells
4. Cryopreservation: Freezing & thawing of human cancer cell lines
5. Genomic DNA isolation from cancer cell lines,
6. Genomic DNA estimation
7. Genomic DNA agarose gel electrophoresis
8. Total RNA extraction from human cancer cell lines
9. cDNA synthesis from RNA (Reverse Transcription),
10. Polymerase Chain Reaction from cDNA (Reverse Transcription-PCR)
11. Agarose gel electrophoresis of PCR product
12. Total protein extraction from human cancer cell lines
13. Protein estimation
14. SDS-PAGE electrophoresis of extracted proteins
15. Coomassie blue staining of protein gel

This workshop is designed for beginners of all ages who wants to become confidant in lab techniques. This is a practical hands-on workshop to train the candidates in animal cell culture, molecular biology techniques and MTT assay using the human embryonic and cancer cell lines for various experiments. It is supported by lectures on cell culture techniques, molecular biology assays, MTT assay, gene/protein expression/interaction networks in hematopoietic stem cells (HSCs) and cancer cells.