Sheo Mohan Singh

28/07/2026

1-Week Hands-on Workshop on Human/Cancer Cell Culture Techniques & MTT Assay, Delhi. Next batch: 18-24 Aug & 6-12 Sept 2026. Apply now on: https://forms.gle/YaPULvEf4vZo3Lji7, limited seats to provide high quality individual training.

Following techniques will be done hands-on by each candidate:

1. MTT Assay for cell proliferation/cytotoxicity
2. Cell culture techniques, passaging and maintenance of human cancer cell lines
3. Media preparation and counting of cells
4. Cryopreservation: Freezing & thawing of human cancer cell lines
5. Genomic DNA isolation from cancer cell lines,
6. Genomic DNA estimation
7. Genomic DNA agarose gel electrophoresis
8. Total RNA extraction from human cancer cell lines
9. cDNA synthesis from RNA (Reverse Transcription),
10. Polymerase Chain Reaction from cDNA (Reverse Transcription-PCR)
11. Agarose gel electrophoresis of PCR product
12. Total protein extraction from human cancer cell lines
13. Protein estimation
14. SDS-PAGE electrophoresis of extracted proteins
15. Coomassie blue staining of protein gel

This workshop is designed for people of all ages who wants to become confidant in lab techniques. This is a practical hands-on workshop to train the candidates in animal cell culture, molecular biology techniques and MTT assay using the human embryonic and cancer cell lines for various experiments. It is supported by lectures on cell culture techniques, molecular biology assays, MTT assay, gene/protein expression/interaction networks in hematopoietic stem cells (HSCs) and cancer cells.

27/07/2026

1-Month Training on Biotechnology, Animal Cell Culture and Cancer Biology, Delhi. Next batches on 01 Aug and 01 Sept 2026. Apply on https://forms.gle/MgWXWHehufxaQodA8, limited seats to provide high quality individual training.

Following techniques (hands-on by each candidate) will be done during the program:

Molecular Biology and Cell Culture Lab:
1. MTT Assay for cell proliferation/cytotoxicity
2. Cell culture techniques, passaging and maintenance of human cancer cell line
3. Media preparation and counting of cells
4. Cryopreservation: Freezing & thawing of human cancer cell line
5. Genomic DNA isolation from cancer cell line,
6. Genomic DNA estimation
7. Genomic DNA agarose gel electrophoresis
8. Total RNA extraction from human cancer cell line
9. cDNA synthesis from RNA (Reverse Transcription),
10. Polymerase Chain Reaction from cDNA (Reverse Transcription-PCR)
11. Agarose gel electrophoresis of PCR product
12. Total protein extraction from human cancer cell line
13. Protein estimation
14. SDS-PAGE electrophoresis of extracted proteins
15. Coomassie blue staining of SDS-PAGE gel
16. Induction of proliferation and differentiation in human cancer cell line
17. Cytospin slide preparation of hematopoietic cells
18. May-Gruenwald Giemsa Staining of cytospin slides of hematopoietic cells and visualizing morphology of differentiating cells
19. Plasmid DNA isolation
20. Plasmid DNA gel electrophoresis
21. Plasmid DNA gel extraction
22. Restriction digestion
23. Ligation
24. Agarose gel electrophoresis of restriction digested and ligated DNA
25. Preparation of competent cells
26. Transformation and plating on LBA plates

Bioinformatics tools for the above techniques:
27. DNA databases
28. Sequence Analysis: Pairwise sequence alignment, Multiple sequence alignment, BLAST tools
29. Designing of PCR primers,
30. Restriction analysis, restriction maps, plasmid maps,
31. Searching for homologous and paralogous sequences,
32. Promoter analysis, Transcription factor databases,
33. Whole genome data visualisation: browsing genomic data with the help of NCBI Genome viewer or UCSC Genome browser.

This program is designed for people of all ages who wants to become confidant in lab techniques. This is a practical hands-on workshop to train the candidates in animal cell culture, molecular biology techniques and MTT assay using the human embryonic and cancer cell lines for various experiments. It is supported by lectures on cell culture techniques, molecular biology assays, MTT assay, gene/protein expression/interaction networks in hematopoietic stem cells (HSCs) and cancer cells.

25/07/2026

6-Days Hands-on Workshop on Molecular Biotechnology, PCR and SDS-PAGE, Delhi, Next batch on 25-30 Aug & 16-21 Sept 2026. Apply on: https://forms.gle/xFAvyzzxpa2jLezx5, limited seats for high quality individual training.

Following techniques will be done real hands-on by each candidate:
Recombinant DNA Technology Lab:
1. Plasmid DNA isolation
2. Plasmid DNA agarose gel electrophoresis
3. DNA extraction from agarose gel
4. Restriction digestion of DNA
5. Ligation of DNA
6. Agarose gel electrophoresis of digested and ligated DNA
7. Preparation of competent cells
8. Transformation of plasmid DNA into competent cells
9. Plating of transformed cells on LB Agar plates
10. Polymerase Chain Reaction (PCR)
11. Agarose gel electrophoresis of PCR product
12. SDS-PAGE electrophoresis of proteins
13. Coomassie staining of protein gel

Bioinformatics supporting tools for the above techniques:
1. DNA databases
2. Sequence Analysis: Pairwise sequence alignment, Multiple sequence alignment, BLAST tools
3. Designing of PCR primers,
4. Restriction analysis, restriction maps, plasmid maps,
5. Searching for homologous and paralogous sequences,
6. Promoter analysis,
7. Whole genome data visualisation: browsing genomic data with the help of NCBI Genome viewer or UCSC Genome browser.

This workshop is designed for people of all ages who wants to become confidant in lab techniques. It is a real hands-on training on the topics mentioned. Major focus is on the lab practicals. It will be supported by lectures in such a manner that it would help a biologist to understand the bioinformatics tools helpful in the lab and a bioinformatics person to understand the practical biotech lab techniques.

Lab Fees: Rs. 6500

24/07/2026

1-Week Hands-on Workshop on Human/Cancer Cell Culture Techniques & MTT Assay, Delhi, 18-24 Aug & 6-12 Sept 2026. Apply now on: https://forms.gle/YaPULvEf4vZo3Lji7, limited to 10 candidates to provide high quality individual training.

Following techniques will be done hands-on by each candidate:

1. MTT Assay for cell proliferation/cytotoxicity
2. Cell culture techniques, passaging and maintenance of human cancer cell lines
3. Media preparation and counting of cells
4. Cryopreservation: Freezing & thawing of human cancer cell lines
5. Genomic DNA isolation from cancer cell lines,
6. Genomic DNA estimation
7. Genomic DNA agarose gel electrophoresis
8. Total RNA extraction from human cancer cell lines
9. cDNA synthesis from RNA (Reverse Transcription),
10. Polymerase Chain Reaction from cDNA (Reverse Transcription-PCR)
11. Agarose gel electrophoresis of PCR product
12. Total protein extraction from human cancer cell lines
13. Protein estimation
14. SDS-PAGE electrophoresis of extracted proteins
15. Coomassie blue staining of protein gel

This workshop is designed for people of all ages who wants to become confidant in lab techniques. This is a practical hands-on workshop to train the candidates in animal cell culture, molecular biology techniques and MTT assay using the human embryonic and cancer cell lines for various experiments. It is supported by lectures on cell culture techniques, molecular biology assays, MTT assay, gene/protein expression/interaction networks in hematopoietic stem cells (HSCs) and cancer cells.

Lab Fees: Rs. 11000

Deposit the fees as described below and submit this form to confirm participation.

18/07/2026

1-Month Training Program on Biotechnology, Animal Cell Culture and Cancer Biology, Delhi, Batches starting on 01 Aug and 01 Sept 2026. Apply on https://forms.gle/MgWXWHehufxaQodA8, we take only 10 candidates and provide high quality individual training.

Following techniques (hands-on by each candidate) will be done during the program:

Molecular Biology and Cell Culture Lab:
1. MTT Assay for cell proliferation/cytotoxicity
2. Cell culture techniques, passaging and maintenance of human cancer cell line
3. Media preparation and counting of cells
4. Cryopreservation: Freezing & thawing of human cancer cell line
5. Genomic DNA isolation from cancer cell line,
6. Genomic DNA estimation
7. Genomic DNA agarose gel electrophoresis
8. Total RNA extraction from human cancer cell line
9. cDNA synthesis from RNA (Reverse Transcription),
10. Polymerase Chain Reaction from cDNA (Reverse Transcription-PCR)
11. Agarose gel electrophoresis of PCR product
12. Total protein extraction from human cancer cell line
13. Protein estimation
14. SDS-PAGE electrophoresis of extracted proteins
15. Coomassie blue staining of SDS-PAGE gel
16. Induction of proliferation and differentiation in human cancer cell line
17. Cytospin slide preparation of hematopoietic cells
18. May-Gruenwald Giemsa Staining of cytospin slides of hematopoietic cells and visualizing morphology of differentiating cells
19. Plasmid DNA isolation
20. Plasmid DNA gel electrophoresis
21. Plasmid DNA gel extraction
22. Restriction digestion
23. Ligation
24. Agarose gel electrophoresis of restriction digested and ligated DNA
25. Preparation of competent cells
26. Transformation and plating on LBA plates

Bioinformatics tools for the above techniques:
27. DNA databases
28. Sequence Analysis: Pairwise sequence alignment, Multiple sequence alignment, BLAST tools
29. Designing of PCR primers,
30. Restriction analysis, restriction maps, plasmid maps,
31. Searching for homologous and paralogous sequences,
32. Promoter analysis, Transcription factor databases,
33. Whole genome data visualisation: browsing genomic data with the help of NCBI Genome viewer or UCSC Genome browser.

This program is designed for people of all ages who wants to become confidant in lab techniques. This is a practical hands-on workshop to train the candidates in animal cell culture, molecular biology techniques and MTT assay using the human embryonic and cancer cell lines for various experiments. It is supported by lectures on cell culture techniques, molecular biology assays, MTT assay, gene/protein expression/interaction networks in hematopoietic stem cells (HSCs) and cancer cells.

Lab Fees: Rs. 19500

Deposit the fees as described below and submit this form to confirm participation.

15/07/2026

1-Week Hands-on Workshop on Human/Cancer Cell Culture Techniques & MTT Assay, Delhi, 25-31 July and 18-24 August 2026. Apply on: https://forms.gle/YaPULvEf4vZo3Lji7, we take only 10 candidates and provide high quality individual training.

Following techniques will be done hands-on by each candidate:

1. MTT Assay for cell proliferation/cytotoxicity
2. Cell culture techniques, passaging and maintenance of human cancer cell lines
3. Media preparation and counting of cells
4. Cryopreservation: Freezing & thawing of human cancer cell lines
5. Genomic DNA isolation from cancer cell lines,
6. Genomic DNA estimation
7. Genomic DNA agarose gel electrophoresis
8. Total RNA extraction from human cancer cell lines
9. cDNA synthesis from RNA (Reverse Transcription),
10. Polymerase Chain Reaction from cDNA (Reverse Transcription-PCR)
11. Agarose gel electrophoresis of PCR product
12. Total protein extraction from human cancer cell lines
13. Protein estimation
14. SDS-PAGE electrophoresis of extracted proteins
15. Coomassie blue staining of protein gel

This workshop is designed for people of all ages who wants to become confidant in lab techniques. This is a practical hands-on workshop to train the candidates in animal cell culture, molecular biology techniques and MTT assay using the human embryonic and cancer cell lines for various experiments. It is supported by lectures on cell culture techniques, molecular biology assays, MTT assay, gene/protein expression/interaction networks in hematopoietic stem cells (HSCs) and cancer cells.

Lab Fees: Rs. 11000

Deposit the fees as described below and submit this form to confirm participation.

13/07/2026

1-Month Training Program on Biotechnology, Animal Cell Culture and Cancer Biology, Delhi, Batches starting on 18 July, 01 Aug, and 01 Sept 2026. Apply on https://forms.gle/MgWXWHehufxaQodA8, we take only 10 candidates and provide high quality individual training.

Following techniques (hands-on by each candidate) will be done during the program:

Molecular Biology and Cell Culture Lab:
1. MTT Assay for cell proliferation/cytotoxicity
2. Cell culture techniques, passaging and maintenance of human cancer cell line
3. Media preparation and counting of cells
4. Cryopreservation: Freezing & thawing of human cancer cell line
5. Genomic DNA isolation from cancer cell line,
6. Genomic DNA estimation
7. Genomic DNA agarose gel electrophoresis
8. Total RNA extraction from human cancer cell line
9. cDNA synthesis from RNA (Reverse Transcription),
10. Polymerase Chain Reaction from cDNA (Reverse Transcription-PCR)
11. Agarose gel electrophoresis of PCR product
12. Total protein extraction from human cancer cell line
13. Protein estimation
14. SDS-PAGE electrophoresis of extracted proteins
15. Coomassie blue staining of SDS-PAGE gel
16. Induction of proliferation and differentiation in human cancer cell line
17. Cytospin slide preparation of hematopoietic cells
18. May-Gruenwald Giemsa Staining of cytospin slides of hematopoietic cells and visualizing morphology of differentiating cells
19. Plasmid DNA isolation
20. Plasmid DNA gel electrophoresis
21. Plasmid DNA gel extraction
22. Restriction digestion
23. Ligation
24. Agarose gel electrophoresis of restriction digested and ligated DNA
25. Preparation of competent cells
26. Transformation and plating on LBA plates

Bioinformatics tools for the above techniques:
27. DNA databases
28. Sequence Analysis: Pairwise sequence alignment, Multiple sequence alignment, BLAST tools
29. Designing of PCR primers,
30. Restriction analysis, restriction maps, plasmid maps,
31. Searching for homologous and paralogous sequences,
32. Promoter analysis, Transcription factor databases,
33. Whole genome data visualisation: browsing genomic data with the help of NCBI Genome viewer or UCSC Genome browser.

This program is designed for people of all ages who wants to become confidant in lab techniques. This is a practical hands-on workshop to train the candidates in animal cell culture, molecular biology techniques and MTT assay using the human embryonic and cancer cell lines for various experiments. It is supported by lectures on cell culture techniques, molecular biology assays, MTT assay, gene/protein expression/interaction networks in hematopoietic stem cells (HSCs) and cancer cells.

Lab Fees: Rs. 19500

Deposit the fees as described below and submit this form to confirm participation.

10/07/2026

6-Days Hands-on Workshop on Molecular Biotechnology, PCR and SDS-PAGE, Delhi, 18-23 July and 25-30 Aug 2026. Apply on: https://forms.gle/xFAvyzzxpa2jLezx5, we take only 10 candidates and provide high quality individual training.

Following techniques will be done real hands-on by each candidate:
Recombinant DNA Technology Lab:
1. Plasmid DNA isolation
2. Plasmid DNA agarose gel electrophoresis
3. DNA extraction from agarose gel
4. Restriction digestion of DNA
5. Ligation of DNA
6. Agarose gel electrophoresis of digested and ligated DNA
7. Preparation of competent cells
8. Transformation of plasmid DNA into competent cells
9. Plating of transformed cells on LB Agar plates
10. Polymerase Chain Reaction (PCR)
11. Agarose gel electrophoresis of PCR product
12. SDS-PAGE electrophoresis of proteins
13. Coomassie staining of protein gel

Bioinformatics supporting tools for the above techniques:
1. DNA databases
2. Sequence Analysis: Pairwise sequence alignment, Multiple sequence alignment, BLAST tools
3. Designing of PCR primers,
4. Restriction analysis, restriction maps, plasmid maps,
5. Searching for homologous and paralogous sequences,
6. Promoter analysis,
7. Whole genome data visualisation: browsing genomic data with the help of NCBI Genome viewer or UCSC Genome browser.

This workshop is designed for people of all ages who wants to become confidant in lab techniques. It is a real hands-on training on the topics mentioned. Major focus is on the lab practicals. It will be supported by lectures in such a manner that it would help a biologist to understand the bioinformatics tools helpful in the lab and a bioinformatics person to understand the practical biotech lab techniques.

Deposit the fees as described below and submit this form to confirm participation.

04/07/2026

1-Week Hands-on Workshop on Human/Cancer Cell Culture Techniques & MTT Assay, Delhi, 25-31 July 2026, Apply on: https://forms.gle/qXXArKTK5YjQppgr9

Following techniques will be done hands-on by each candidate:

1. MTT Assay for cell proliferation/cytotoxicity
2. Cell culture techniques, passaging and maintenance of human cancer cell lines
3. Media preparation and counting of cells
4. Cryopreservation: Freezing & thawing of human cancer cell lines
5. Genomic DNA isolation from cancer cell lines,
6. Genomic DNA estimation
7. Genomic DNA agarose gel electrophoresis
8. Total RNA extraction from human cancer cell lines
9. cDNA synthesis from RNA (Reverse Transcription),
10. Polymerase Chain Reaction from cDNA (Reverse Transcription-PCR)
11. Agarose gel electrophoresis of PCR product
12. Total protein extraction from human cancer cell lines
13. Protein estimation
14. SDS-PAGE electrophoresis of extracted proteins
15. Coomassie blue staining of protein gel

This workshop is designed for people of all ages who wants to become confidant in lab techniques. This is a practical hands-on workshop to train the candidates in animal cell culture, molecular biology techniques and MTT assay using the human embryonic and cancer cell lines for various experiments. It is supported by lectures on cell culture techniques, molecular biology assays, MTT assay, gene/protein expression/interaction networks in hematopoietic stem cells (HSCs) and cancer cells.

Submit this form and deposit the fees after receiving the confirmation email.