21/03/2023
Research on an innovative dimension of protein packaging foreseeing the development of more robust biocatalysts that can be used at high temperatures has been published by our team led by Prof. Nataraj Sk and Dr. Dibyendu Mondal.
ಈ ಸಂಶೋಧನೆಯು ಪ್ರೋಟೀನ್ ಪ್ಯಾಕೇಜಿಂಗ್ನ ನವೀನ ಆಯಾಮವನ್ನು ಒಳಗೊಂಡಿರುವ ಕಾರ್ಯತಂತ್ರವನ್ನು ಅನಾವರಣಗೊಳಿಸುತ್ತದೆ, ಇದು ಹೆಚ್ಚಿನ ತಾಪಮಾನದಲ್ಲಿ ಬಳಸಬಹುದಾದ ಹೆಚ್ಚು ದೃಢವಾದ ಜೈವಿಕ ಕ್ಯಾಟಲಿಸ್ಟ್ಗಳ ಅಭಿವೃದ್ಧಿಯನ್ನು ಊಹಿಸುತ್ತದೆ.
We proudly congratulate the author, Dr. Sachin M Shet and all the co-authors, Sarath Kumar Thayallath, Meena Bisht, Matheus M. Pereira, João A. P. Coutinho for this publication!
Herein, a new protein engineering strategy with improved efficacy is demonstrated through judicious task-specific functionalization of Cyt C with quantum dots (QDs) and ionic liquids (ILs). Mn2+ doped ZnS QD and ILs ([Cho][Ac]; [Cho][Dhp]) were concomitantly used to decorate Cyt C, which was characterized using various analytical tools. The peroxidase activity at room temperature of engineered Cyt C (Cyt C-QD-IL) increased markedly (1.2 to 3.5-fold) as compared to that for bare Cyt C, Cyt C with QD, and Cyt C with ILs. Further, Cyt C-QD-IL showed better catalytic activity under various stresses such as high temperature (110 °C), presence of a chemical denaturant (6 M GuHCl), high oxidative stress (30 min H2O2), and presence of proteases.
Enhancing the structural stability and catalytic activity of Cytochorme c (Cyt C) against harsh process conditions would boost its use in biocatalysis. Herein, a new protein engineering strategy with improved efficacy is demonstrated through judicious task-specific functionalization of Cyt C with qu...