20/09/2019
In this movie and in the zoom-in below you can see T-Cells killing cancer cells.
The cancer cell line is MC38-OVA, a transduced colon cancer cell line that expresses the ovalbumin (OVA) model antigen.
T-cells, coming from OT-I mice, carry a transgenic T-cell receptor responsive to OVA residues 257-264 (SIINFEKL peptide) in the context of the MHC I H2kb.
In this experiment, the T-cells that were activated in the first experiment and that are now called “effectors”, are incubated with MC38-OVA cancer cells. Upon recognition of their target (the OVA residues on the MHC I H2kB of the cancer cells), T-cells induce the killing of the cancer cells. However, this does not happen all the time and many cancer cells survive.
In this movie (killing assay) you can see T-Cells and cancer cells. The cancer cell line is MC38-OVA, a transduced colon cancer cell line that expresses the ovalbumin…
