ZhongKe Publishing

Selenium nanoparticles and glutathione synergistically enhance soybean salt tolerance by activating the JA pathway and arbutin-mediated rhizosphere microbiota

In a study published in aBIOTECH, researchers developed a synergistic nano-biostimulant (SeG) that enhances soybean salt tolerance. The research uncovers a comprehensive mechanism in which SeG activates the jasmonic acid (JA) pathway, promotes the secretion of arbutin, and recruits beneficial rhizosphere microbiota, collectively helping the plant to combat salt stress.

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https://www.sciencedirect.com/science/article/pii/S2662173826000032

Oxidation of ABI4 by RBOHD-derived ROS integrates redox signaling into ABA and drought stress responses
In a paper published on aBIOTECH, the authors reveal that the transcription factor ABI4 acts upstream of RBOHD to regulate ABA-induced ROS production, while RBOHD-derived ROS in turn promote ABI4 sulfenylation at Cys250, enhancing its DNA-binding and transcriptional activity—forming a redox-dependent feedback loop that fine-tunes stomatal closure and drought tolerance in plants.

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https://www.sciencedirect.com/science/article/pii/S2662173826000512

A pathogen-induced translational shift enhances plant disease resistance without obvious fitness costs
In a paper published on aBIOTECH, Prof. Xueping Zhou’s team at Zhejiang University established
a technical system for the specific regulation of resistance gene expression via protein translation reprogramming.

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https://www.sciencedirect.com/science/article/pii/S2662173826000391

Engineering high-efficiency mutants of the transposase ISDra2 TnpB via protein-generative models and energy-optimized design

In a paper published on aBIOTECH, the authors developed a method termed TnpB-GMRE, which integrates a generative protein model for TnpB with a virtual screening pipeline based on the minimum recovery rate and energy minimization, to obtain a TnpB-TD mutant with enhanced editing activity.

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https://www.sciencedirect.com/science/article/pii/S266217382600041X

Physicochemical Dual Cross-Linked Multifunctional Conductive Organohydrogel Sensors for Fireworks Burn Wound Healing and Intelligent Real-Time Monitoring

In a paper published in Polymer Science & Technology, an international team of scientists
developed a multifunctional conductive hydrogel (P-EPL/CCT) hydrogel flexible sensor using a physical-chemical dual cross-linking approach involving poly(vinyl alcohol) (PVA), gallic acid grafted chitosan (CS−GA), tannic acid (TA), eggshell membrane (ESM), lysozyme, and 4am-PEG-MAL for emergency cooling and wound healing following fire-works-related skin burns. The organohydrogel sensor exhibits impressive mechanical properties, such as a maximum stress of 2.15 MPa and an elongation of 605%, along with antifreeze resistance down to −39.5 °C, antimicrobial properties with bacterial inhibition exceeding 96.5%, and cytocompatibility. Additionally, serving as strain sensor with high sensitivity (GF = 1.14 at 100% strain) and rapid response times, it can effectively monitor human movement signals. The developed organohydrogel demonstrates the ability to accelerate skin healing, promote angiogenesis, and reduce scarring. Moreover, it is utilized for monitoring finger joint injuries and employing machine learning-assisted electrical signals for intelligent wound healing monitoring and protection. This study introduces a flexible device that combines multiple functionalities, showing promise for diverse applications in the biomedical field. This study is led by Chuang Du (Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China), Weiwei Liu (Stomatological Hospital, Jilin University, Changchun 130021, China) and Lei Wang (Key Laboratory of Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun 130023, China).

Figure: Structural design of organohydrogels and schematic representation of burn modelling on the back of a mouse and skin wound recovery.

Deciphering the dynamics of active autonomous terminal inverted repeat transposons in the plant kingdom

In a paper published on aBIOTECH, the authors constructed a comprehensive atlas of active autonomous terminal inverted repeat (TIR) transposons across 1,007 plant genomes. The study reveals the lineage-specific distribution of these elements, their interspecific and intraspecific diversification as well as the adaptive mechanisms.

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https://www.sciencedirect.com/science/article/pii/S2662173825002164

Double-fluorescent proteins enable robust maternal haploid identification in wheat

In a paper published on aBIOTECH, the authors developed an efficient haploid identification (HID) system for wheat by seamlessly integrating a two-fluorescent protein-based HID toolbox with a MATRILINEAL (MTL) mutant haploid induction (HI) wheat line. This system enables efficient HI and utilizes dual-fluorescence screening, offering high accuracy while being independent of the genetic background of the recipient material.

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https://www.sciencedirect.com/science/article/pii/S2662173825002231

High levels of ADAR overexpression induce abundant and stochastic off-target RNA editing in rice protoplasts

In a paper published on aBIOTECH, the authors investigated the off-target effects of ADAR-mediated RNA editing in rice protoplasts, revealing that high levels of ADAR can induce abundant stochastic RNA editing and protoplast-based assessments of RNA editors require rigorous validation.

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https://www.sciencedirect.com/science/article/pii/S2662173825002140

GmWOX1 regulates the mediolateral polarity of compound leaves in soybean

In a paper recently published on aBIOTECH, the authors combined spatiotemporal expression analysis and CRISPR/Cas9 genome editing to dissect the roles of four WOX1 homologs in soybean. This study revealed their functional redundancy, and showed that triple mutants develop narrow leaves. GmWOX1a could rescue the leaf phenotype of lam1 mutant in Nicotiana sylvestris.

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https://www.sciencedirect.com/science/article/pii/S2662173825002188

BacPhase: Long-insert paired-end sequencing for bin marker construction and genome phasing

In a paper published on aBIOTECH, the authors introduce BacPhase, a sequence-based phasing method that uses restriction enzyme-digested and self-ligated BACs followed by PCR and PacBio HiFi sequencing. It generates high-resolution bin markers, enabling precise haplotype resolution in polyploid plants like potato without Hi-C or physical maps, significantly improving genome scaffolding and supporting breeding applications.

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https://www.sciencedirect.com/science/article/pii/S266217382500219X

A strand and whole genome duplication–aware collinear gene identification tool

In a paper published on aBIOTECH, the authors leveraged the longest-path algorithm in conjunction with the species’ whole-genome duplication history to precisely control alignment depth and identify collinear gene pairs. They further conducted a differential analysis of inverted versus non-inverted genes within collinear blocks to uncover potential functional and evolutionary distinctions.

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https://www.sciencedirect.com/science/article/pii/S2662173825002152