Laboratório Dalton de Espectrometria de Massas

One of the biggest challenges in protein characterization with cross-linking/mass spectrometry is to find chemicals which can react at room temperature (or lower), physiological pH and without disturbing the protein native structure. Cross-linking chemistry has been limited to the availability of nucleophilic residues (especially Lysines) on the protein surface which reacts with NHS-based cross-linkers.

In our recent Analytical Chemistry publication, we show a new cross-linking chemistry which targets acidic residues by the use of commercially available diamines. Also, it can simultaneously form zero-length species. For this reason, it is named Xplex (after multiplex cross-linking chemistry). This new methodology finds immediate application in protein structure characterization.

10.1021/acs.analchem.7b05135

2018 started with a fantastic news: our paper on the methodology to solve the solution structure of homodimers was just published in Nature Protocols ! This is the result of a continuous effort to make use of advanced mass spectrometry techniques to understand the structure and function of proteins and complexes.
This is another great outcome from a very fruitful collaboration with Paulo Costa Carvalho and Diogo Borges on the computational proteomics field.
Congrats to all authors!

Last week, two of ours members started working in reference international laboratories doing part of their PhD thesis. Tatiani Brenelli is currently working with Prof. Andrea Sinz, one the pioneers in cross-linking/MS and one of the most active researchers in the field. Állan Ferrari is now working in Prof. David Baker´s lab, a leading protein structure modeling group. Good luck and success to you both !

2017 has been an incredible year for Dalton lab ! The PhD thesis of Diogo Borges was selected for honorable mention (runner up) as the best thesis of 2016 by Capes agency !
This thesis was advised by Paulo Costa Carvalho and co-advised by Fabio Gozzo. Diogo developed SIM-XL, a software for identification of cross-linked peptides used in structural proteomics (http://patternlabforproteomics.org/sim-xl), and SIM-XL has become a landmark in the field, with more than 3.000 downloads.
Congrats, Diogo !

SIM-XL - Spectrum Identification Machine for XL peptides

Dalton's lab barbecue!

Dalton MS Lab talks in the BrMass conference.

Covalent labeling and chemical cross-linking oral section. ASMS 2016

The boss also makes birthday! (Photo for everyone look skinny)

Happy birthday Hugo

Laboratório Dalton na ASMS 2015 - St. Louis

Ranking of scientists in Brazilian Institutions according to their GSC | Ranking Web of Universities This edition was built with data collected during the first week of February 2015 from the public profiles of researchers working in Brazilian (Brazil, South America) institutions. It is a BETA Ranking of the scientists according to their declared (voluntarily) presence in the Google Schol…