Servicio de Anatomía Patológica Veterinaria

Patient canine, Frisé bug, male, 9 years old.

MV. John Pablo Reale

Large subcutaneous mass attached to deep planes, located in ventral region of the neck, and moving to the trachea.
Estimated evolution time: 9 months.

On the background of erythrocytes is abundant population composed of rounded epithelial cells, isolated and grouped into colgajos, and often forming acinar structures. These cells possess low degree of anisocytosis and anisokaryosis, fine chromatin round nucleus, regular small nucleolus, and little amount of slightly basophile basophile of undefined edges. Presence of occasional cells with cariomegaly. Also visualizes eosinophilic fibrillular extracellular material associated with the pendings. Absence of colloid material or tyrosine granules in this sample

Cytopathology diagnosis
GLANDULAR EPITELIAL NEOPLAR MALIGNA COMPATIBLE WITH CARCINOMA TYROIDEO

Canine patient Yorkshire terrier male, 16 years old.

Scaly papilloma of the pe**le mucosa.

MV. John Pablo Reale

Patient canine, mestizo, male, 1 year and a half.
Subcutaneous mass located in the left side area of the neck, approximately 3 cm in diameter and soft consistency.
Discovered about 2 months ago.
She got a lot better with drug therapy, but it got worse by suspending her.

FNA was made and 3 cc of serosanguinolent liquid were extracted, of cloudy consistency.

In cytology, multiple granulomas can be seen by foreign body, with abundant epithelioid macrophages and periphils arranged around structures in the shape of " shark teeth " or " cat nails ".

I initially made a diagnosis of Severe PIOGRANULOMATOGRANULOMATOUS INFLAMATOUS BY VEGETAL BODY, as the structures described structures were very similar to the thorns of roses, among other plants.

After sharing microscopic images with several colleagues, and with the priceless information provided by Lee, Schlicher Baker and Bau, I modified the diagnosis to:

Severe PIOGRANULOMATORY INFLAMATORY PROCESS BY MOSCA LARVE FRAGMENTS

Asking the owner about the possibility that his dog had previously had miasis, he said the dog had been bitten by another 7 months ago, and that he developed a myasis that was treated in another veterinary clinic.

If the entire larvae are not managed to remove the infested tissue, their remains generate a persistent reaction to a strange body.

MV Santiago Kujman and Silvana Claudia Pepa

While in other countries, miasis by Cuterebra spp (family Oestridae) are common, in our country we are frequently found in the summer with miasis caused by the family Calliphoridae. The larvae of this family of flies are characterized by presenting around their body rings of parallel or irregular pigmented thorns. The common species in our area are Cochliomyia hominivorax and Lucilia sericata also called Phaenicia sericata).

C. C. hominivorax is a forced parasite, well known for its ability to invasion and destruction of tissues, including bone tissue, causing deep wounds that can lead to death. Its larvae develop in fresh fabrics of domestic, wild and man animals producing severe cases of myasis. It is considered one of the most important causes of economic losses in livestock breeding in Central and South America, mainly in Argentina.

P. sericata (L. Sericata), producer of animal and human myasis, corresponds to the group of optional flies, with larvae growing in necrotic fabrics and occasionally invade healthy fabrics. By its necrobiontophagous nature prefers to feed on dead tissues ignoring healthy tissue, although certain strains have shown to have the ability to invade living tissues when the amount of necrotic material is scarce.

• Mulieri, Pablo. (2003). The distribution of the Calliphoridae in Argentina (Diptera). Argantina Entomological Society Magazine. 62. 81-106.

• Visciarelli, Elena, Costamagna, Sixto, Lucchi, Leandro, & Basabe, Norma. (2007). Human Miasis in Bahía Blanca, Argentina: Period 2000 / 2005. Neotropical Entomology, 36 (4), 605-611. https://doi.org/10.1590/S1519-566X2007000400021

• Florez, Eliana, & Wolff, Marta. (2009). Description and key of immature stadiums of the main species of Calliphoridae (Diptera) of forensic importance in Colombia. Neotropical Entomology, 38(3), 418-429. https://doi.org/10.1590/S1519-566X2009000300019

• Bau-Gaudreault, Liza & Overvelde, Sébastien & Martin, Donald. (2018). What is your diagnosis? Subcutaneous temporal mass from a cat. Veterinary Clinical Pathology. 47. 10.1111/vcp.12601.

Sialocele
Large globular accumuli of mucus and macrophages, some of which contain hematoidine crystals, evidence of prior bleeding.

Equine, Creole, male, 7 years old

Pedunculated mass located in ventral area of the right hemimjaw, of hard consistency, mineralized.
Discovered 1 year ago.

Diagnosis: OSTEOMA

MV. Nicolas Micheli.

Osteomas are benign tumors frequently located in jaw, maxilla, nasal breasts and face bones and skull of equines and bovine animals, although they also occur in other species.
Clinical signs are limited to a deformation of the area of origin or eventually a compression of adjacent structures.

- Olds, Ashleigh & Stewart, Allison & Grimm, Jennifer. (2004). What is your diagnosis? A pedunculated heterogeneous mineral opacity structure is evident ventral to the third premolar on the left mandible. Journal of the American Veterinary Medical Association. 224. 1073-4.

- Fiani, Nadine & Arzi, Boaz & Johnson, Eric & Murphy, Brian & Verstraete, Frank. (2011). Osteoma of the oral and maxillofacial regions in cats: 7 cases (1999–2009). Journal of the American Veterinary Medical Association. 238. 1470-5. 10.2460/javma.238.11.1470.

Canine patient, Shar Pei, female, 10 years old.

Background: 2 years ago the patient was extracted a mass located in left escapular zone. Currently it has multiple dermal and subcutaneous masses spread around this area, armpit and neck, some pedunculated, ulcerated and with variable black pigmentation. Severe adenomegaly of left pre-escapular lymphonodule.

It can be observed abundant population composed of rounded, isolated or grouped cells, with high degree of anisocytosis and anisokaryosis, with round nucleus of thick chromatin, one to two prominent irregular nucleoli and moderate amount of slightly basophile cytoplasm containing very few green granules - blackish. Presence of binucleate cells, cariomegaly and numerous atypical mitotic figures. Numerous scattered melanophages are also dispersed.

Cytopathological diagnosis:
MELANOMA CUTANOMA DISEMINED WITH METASTASIC LYMPHODULE

MV. Martin Aureggi

Cytohistopathologic correlation of Fibroepithelial Mamaria Feline Hyperplasia.

Glandular epithelial cells are embedded in a dense fibrous stroma.

Macroscopic photo from: Payan Carreira, Rita. (2013) Feline Mammary Fibroepithelial Hyperplasia: A Clinical Approach. 10.5772 / 55550.

Patient canine, half-breed, geronte.

Cryptorquid.
Sertoli cell tumor in testicle located on inguinal channel.
Feminization syndrome product of hyperrestrogenism, with semialopecia and hirsute fur that respects the head and limbs, with gynecomastia and fo****in pendulum.

MV. Rosa Elizabeth Perez.

Feline patient Siamese male, 6 years old.

Metastatic calcification of interdigital spaces and pads due to chronic kidney failure.

MV. Pablo Parodi.

The term "metastatic" in this case is not necessarily related to neoplasms.
Skin calcifications can be classified into distróphic, metastatic, iatrogenic or idiopathic.
Any disorder that causes hypercalcaemia and / or hyperphosphatemia (hypervitaminosis D, lymphomas, multiple myeloma, carcinomas, kidney diseases, systemic blastomycosis) can be responsible for metastatic calcification.

W Bertazzolo, L Toscani, S Calcaterra, L Crippa, M Caniatti, U Bonfanti. Clinicopathological findings in five cats with paw calcification. Journal of Feline Medicine & Surgery, Volume 5, Issue 1, 2003, Pages 11-17.

Canine patient, Beagle, male, 15 years old

Haematuria for four months, associated with the presence of a vesical mass.

Transitional cell carcinoma

Notice the bodies of Melamed-Wolinska, characteristic of this neoplasm. Not seen in every CST, but here they are obvious.

Patient canine, half-breed, female, 12 years old.

MV. Estefania Mompart

Subcutaneous mass located in left metatarsal plant zone, of soft consistency, approximately 35 mm in diameter, semi-allopecic and erythematous, bleeding to puncture. Estimated evolution time: 1 month.

DIAGNOSTIC CYTOPATOLOGICAL
SARCOMA OF WHITE WNITE COMPATIBLE WITH TUMOR OF THE PERIVASCULAR PARED

All soft tissue sarcomas present the same biological behavior, including:
- Peripheral nerve pod tumors (but not brachial plexus),
- Fibrosarcoma,
- Mixosarcoma,
- Liposarcoma,
- Perivascular wall tumors,
- Pleomorphic Sarcoma (Evil Fibrous Histiocytoma),
- Malignant mesenchimoma, and
- Indifferentiated sarcoma.

Perivascular wall tumors include:
- Hemangiopicitoma
- Myopicytoma
- Glomic tumor
- Angioleioma-Angioleiomyosarcoma
- Angiomiofibroblastoma
- Angiofibros

Cytological evaluation does not allow a distinction between the different types of tumors of the perivascular wall, but the combination of moderate to high cellularity, cohesive fusiform cells, the presence of capillaries and the observation of multinucleate cells in crown should be considered typical of a tumor of the perivascular wall, as the coexistence of these cytological characteristics is unique and has not been observed in other canine skin tumors of fusiform cells, such as melanoma of fusiform cells, angiosarcoma and histiocytic sarcoma, among others.

References:
- Caniatti, Mario & Ghisleni, Gabriele & Ceruti, Roberta & Roccabianca, Paola & Scanziani, E. (2001). Cytological features of dogs hemangiopericytoma at the end needle aspiration biopsy. The Veterinary record. 149. 242-4. 10.1136 / vr. 149.8.242.
- Avallone, Giancarlo & Helmbold, P & Caniatti, Mario & Stefanello, Damiano & Nayak, Ramesh & Roccabianca, Paola. (2007). The Spectrum of Canine Cutaneous Perivascular Wall Tumors: Morphologic, Phenotypic and Clinical Characterization. Veterinary pathology. 44. 607-20. 10.1354/vp.44-5-607.

Canine patient, medium half-breed, female, 9 years old.

Dermal injury located in the left carpal side area, approximately 2 x 1 cm, semi-alloecic and wet surface, soft to palpation.
They are made extended from scraping and PAF.

Note: Patient records history of severe ulcerated injury in proximity to this injury, which she solved completely with medication, leaving a large scar in the area.

On the background of erythrocytes, abundant population composed of preserved neutrophils, with marked proportion of intensely vacuolated cytoplasm macrophages, some of them binucleated or multinucleated. These inflammatory cells are associated with frequent keratinized epithelial cells anucleated, isolated or grouped into pendings. Absence of microorganisms or malignancy characteristics in this sample.

Cytopathological diagnosis
SUPURATIVE INFLAMATIVE, CHRONIC, Severe (SUGEST FORUNCULOSIS)

Some chronic skin injuries tend to develop cystic and tortuous follicles with abundant keratin in their light, and whose wall sometimes breaks down, causing a "strange body reaction" to such free keratin; to this breakdown of the pilous follicle and the subsequent inflammatory reaction is called forunculosis. Sometimes, in cytology, inflammatory cells can be seen surrounding keratin remains or anucleated keratin cells. In this cytological table, multinucleated macrophages should not be confused with malignant cells.