Lab Notes with RS

Blood Gas Analysis (ABG) – Quick Gists

Proper specimen handling is VERY important in Blood Gas Analysis because pH, pCO₂, and pO₂ can change quickly after collection.

📌 Specimen Collection
• Use a heparinized syringe
• Remove air bubbles immediately
• Mix sample well after collection
• Transport to the lab ASAP

Sample Stability
• Stable for 30 minutes at room temperature
• Stable for 1 hour if placed on ice

❌ Rejection Criteria
• Delayed transport beyond stability time
• Clots
• Air bubbles in syringe

Factors Affecting Blood Gas Results

Air Bubbles in Syringe:
⬆️ pH
⬇️ pCO₂
⬆️ pO₂

Sample Sitting >30 mins (Not on Ice):
⬇️ pH
⬆️ pCO₂
⬇️ pO₂

Why does this happen?
Cells continue metabolism after collection:
• Oxygen is consumed → pO₂ decreases
• CO₂ is produced → pCO₂ increases
• Increased acidity → pH decreases

Critical Values
• pH < 7.2 or > 7.6
• pO₂ < 50 mmHg
• pCO₂ > 55 mmHg

Small pre-analytical errors can lead to major clinical impact in ABG interpretation.

✨ How to Ace Your ASCPi Exam! ✨

Preparing for the ASCPi exam? The secret is simple: Study smart, not just hard. 🔬

Here is your quick roadmap to success:

🔹 Focus on High-Yield Topics: Spend the most time on Blood Banking , Quality Control, Hematology, Clinical Chemistry patterns, and Microbiology. 🧪
🔹 Understand the "Why": Practice MCQs daily. Don't just memorize the correct answer—understand why it's right.
🔹 Connect the Dots: Don't just memorize numbers. Learn how abnormal lab results relate to real diseases. 📊
🔹 Use your exam time wisely: Don't waste too much time on a single tough question during the exam. ⏱️
🔹 Consistency Wins: 30 minutes of daily study is much better than last-minute cramming. 💡

Small daily efforts lead to big success. You've got this, future MLS! 🚀

🧪 To Every Laboratory Professional

Behind every accurate diagnosis is a laboratory professional working with dedication, precision, and care.

Your work may be behind the scenes, but it plays a vital role in patient care every single day.

Keep learning. Keep growing. Your knowledge saves lives. 💙

🧪 What is Hematopoiesis?
⚫️Production
⚪️Differentiation
🔴Maturation &
🔵Life Cycle of Blood cells.


Follow Lab Notes with RS for more ASCP and laboratory science notes ✨

🧪 ASCP Quick Note: HIL Interference in Clinical Chemistry

HIL = Hemolysis • Icteric • Lipemic

These are the major sample interferences in clinical chemistry that may affect analytical accuracy and patient results.

🔴 Hemolysis
• Caused by rupture of red blood cells
• Releases intracellular contents into serum/plasma
• May falsely increase:
→ Potassium (K⁺)
→ LDH
→ AST

🟡 Icteric
• Due to high bilirubin levels
• Serum appears dark yellow
• Bilirubin may interfere with photometric reactions and absorbance readings.

⚪ Lipemic
• Caused by high lipid/triglyceride levels
• Serum appears milky or cloudy
• Can affect light transmission and electrolyte measurements.

⚠️ Always assess sample quality before analysis to ensure accurate laboratory results.

📚 Small concepts like HIL are high-yield topics for ASCP preparation.

Follow Lab Notes with RS for more ASCP and laboratory science notes ✨

🧪 Starting my ASCP journey with Lab Notes with RS ✨

ASCP is not just an exam — it is a step toward improving knowledge, confidence, and professional growth in the medical laboratory field.

📚 Small progress every day is still progress.

Follow this page for:
✔️ ASCP study tips
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