Dr. Haider for sequencing data analysis

Submission of local Iraq isolate in NCBI: The Agaricus bisporus var. bisporus small subunit ribosomal RNA gene were registered after the correspondence of the National Center for Biotechnology Information and obtained accession number and became a reference to Iraq and the Middle East and the world. Ongoing work will add to this set as more type strains are publishedand it is available for download at ID : MZ619063.1 and MZ798947.1
Agaricus bisporus var. bisporus clone NeBa-1 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene, complete sequence; and internal transcribed spacer 2, partial sequence
GenBank: MZ619063.1

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LOCUS MZ619063 675 bp DNA linear PLN 31-JUL-2021
DEFINITION Agaricus bisporus var. bisporus clone NeBa-1 internal transcribed
spacer 1, partial sequence; 5.8S ribosomal RNA gene, complete
sequence; and internal transcribed spacer 2, partial sequence.
ACCESSION MZ619063
VERSION MZ619063.1
KEYWORDS .
SOURCE Agaricus bisporus var. bisporus
ORGANISM Agaricus bisporus var. bisporus
Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina;
Agaricomycetes; Agaricomycetidae; Agaricales; Agaricaceae;
Agaricus.
REFERENCE 1 (bases 1 to 675)
AUTHORS Nedal,W.H. and Ban,T.M.
TITLE The effect of some Green Nanopartical compound on some
physiylogical Genome sequences traits of some dermatophyte fungi
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 675)
AUTHORS Nedal,W.H. and Ban,T.M.
TITLE Direct Submission
JOURNAL Submitted (26-JUL-2021) Teacher, education of Babylon, University
of Karbala college of education for pure science, iraq, Karbala
00964, Iraq
COMMENT # -Data-START # #
Sequencing Technology :: Sanger dideoxy sequencing
# -Data-END # #
FEATURES Location/Qualifiers
source 1..675
/organism="Agaricus bisporus var. bisporus"
/mol_type="genomic DNA"
/variety="bisporus"
/isolate="NeBa-1"
/isolation_source="soil"
/db_xref="taxon:192523"
/clone="NeBa-1"
/country="Iraq"
/collected_by="Nedal Wahab Hameed, Ban Taha Mohamed"
misc_RNA 675
/note="contains internal transcribed spacer 1, 5.8S
ribosomal RNA, and internal transcribed spacer 2"
ORIGIN
1 aggatcatta ttgaattatg ttttctagat gggttgtagc tggctcttcg gagtatgtgc
61 acgcctgtct ggacttcatt ttcatccacc tgtgcacctt ttgtagtctt tttcaggtat
121 tggaggaagt ggtcagccta tcagctcttt gctggatgta aggacttgca gtgtgaaaac
181 agtgttgtcc tttaccttgg ccatggaatc tttttcctgt tagagtctat gttattcatt
241 atactcttag aatgtcattg aatgtcttta catgggctat gcctatgaaa attattatac
301 aactttcagc aacggatctc ttggctctcg catcgatgaa gaccgcagcg aaatgcgata
361 agtaatgtga attgcagaat tcagtgaatc atcgaatctt tgaacgcatc ttgcgctcct
421 tggtattccg aggagcatgc ctgtttgagt gtcattatat tctcaactct ccaatacttt
481 gttgtaaagg agagcttgga ttgtggaggt ttgctggctc cttacttggg gtcagctcct
541 ctgaaatgca ttagcggaat cgtctgcgat ctgccacaag tgtgataact tatctacact
601 ggcgagggga ttgctttctg atgttcagct tctaatcgtc taaggacaat ttcttgaatg
661 cttgacctca aatca
//

Agaricus bisporus var. bisporus clone NeBa-2 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence
GenBank: MZ798947.1

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LOCUS MZ798947 687 bp DNA linear PLN 20-AUG-2021
DEFINITION Agaricus bisporus var. bisporus clone NeBa-2 internal transcribed
spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal
transcribed spacer 2, complete sequence; and large subunit
ribosomal RNA gene, partial sequence.
ACCESSION MZ798947
VERSION MZ798947.1
KEYWORDS .
SOURCE Agaricus bisporus var. bisporus
ORGANISM Agaricus bisporus var. bisporus
Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina;
Agaricomycetes; Agaricomycetidae; Agaricales; Agaricaceae;
Agaricus.
REFERENCE 1 (bases 1 to 687)
AUTHORS Nedal,W.H. and Ban,T.M.
TITLE The effect of some Green Nanopartical compound on some
physiylogical Genome sequences traits of some dermatophyte fungi
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 687)
AUTHORS Nedal,W.H. and Ban,T.M.
TITLE Direct Submission
JOURNAL Submitted (15-AUG-2021) Teacher, education of Babylon, University
of Karbala college of education for pure science, iraq, Karbala
00964, Iraq
COMMENT # -Data-START # #
Sequencing Technology :: Sanger dideoxy sequencing
# -Data-END # #
FEATURES Location/Qualifiers
source 1..687
/organism="Agaricus bisporus var. bisporus"
/mol_type="genomic DNA"
/variety="bisporus"
/isolate="NeBa-2"
/isolation_source="soil"
/db_xref="taxon:192523"
/clone="NeBa-2"
/country="Iraq"
/collected_by="Nedal Wahab Hameed, Ban Taha Mohamed"
misc_RNA 687
/note="contains internal transcribed spacer 1, 5.8S
ribosomal RNA, internal transcribed spacer 2, and large
subunit ribosomal RNA"
ORIGIN
1 ctgcggaagg atcattattg aattatgttt tctagatggg ttgtagctgg ctcttcggag
61 tatgtgcacg cctgtctgga cttcattttc atccacctgt gcaccttttg tagtcttttt
121 caggtattgg aggaagtggt cagcctatca gctctttgct ggatgtaagg acttgcagtg
181 tgaaaacagt gctgtccttt accttggcca tggaatcttt ttcctgttag agtctatgtt
241 attcattata ctcttagaat gtcattgaat gtctttacat gggctatgcc tatgaaaatt
301 attatacaac tttcagcaac ggatctcttg gctctcgcat cgatgaagaa cgcagcgaaa
361 tgcgataagt aatgtgaatt gcagaattca gtgaatcatc gaatctttga acgcatcttg
421 cgctccttgg tattccgagg agcatgcctg tttgagtgtc attatattct caactctccg
481 atactttgtt gtaaaggaga gcttggattg tggaggtttg ctggctcctt acttggggtc
541 agctcctctg aaatgcatta gcggaatcgt ctgcgatctg ccacaagtgt gataacttaa
601 ctacactggc gaggggattg ctttctgatg ttcagcttct aatcgtctaa ggacaatttc
661 ttgaatgctt gacctcaaat caggtag
//

Nedal Wahab Alsady

رسم البروتين

- تحليل نتائج السكونسنك (Sequencing Data Analysis )
- رسم البروتين (Protein Drawing)
- رسم الشجرة الوراثية (Drawing Tree (Phylogenetic
- تسجيل السلالات الوراثية في المنظمة العالمية لبنك الجينات (Submission of NCBI)
- تصميم برايمر